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CAS RN: | Product Number: W0023

WST-8 Reagent [for Cell Proliferation Assay]


Purity:
Synonyms:
Product Documents:
1ML
₩85,100
26   11  
* Please contact our distributor of SEJIN CI Co., Ltd. if you would like to purchase TCI products. The above prices do not include freight cost, customs charge and other charges to the destination. SEJIN CI Co., Ltd. (Phone: 02-2655-2480 / email:  sales@sejinci.co.kr)
* The storage conditions are subject to change without notice.

Supplemental Product Information:

Product Information

  • Volume : 1 mL (100 rxn)

Description

  • This product is supplied as a ready-to-use reagent for the measurement of relative cell number. Reduction of the water-soluble tetrazolium salt WST-8 by the cell membrane-permeable electron carrier 1-MePMS converts it to a highly colored form which can be detected using a plate reader fitted with a 460 nm absorbance filter. As 1-MePMS is itself reduced by intracellular NADH/NADPH, and NADH/NADPH concentration is relatively constant over the course of the cell cycle, the amount of reduced WST-8 and therefore the degree of color change can be used to determine relative cell number,

Direction for Use

    1. Prepare cell samples in a 96 well plate (do not remove medium).
    2. Apply 10 µL of W0023 to each well and incubate for up to 4 hours* at 37°C / 5% CO2.
    3. Measure the absorbance at 460 nm.
  • *Optimal incubation times should be determined for each individual cell line

Storage

  • Store at 4°C. Use within 6 months of receipt.

Product Number W0023
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Heat Sensitive
Specifications
Appearance Light red to Red clear liquid
Cell Number-to-Signal Linearity Test to pass test
Properties (reference)
GHS
Related Laws:
Transport Information:
H.S.code* 3822.19-000
*This code is applied to the products when TCI exports from Japan and not for import in your country.
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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