Sialidase from Arthrobacter ureafaciens

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Synonyms:

• Neuraminidase from Arthrobacter ureafaciens
• α²-3,6,8 Sialidase from Arthrobacter ureafaciens

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Supplemental Product Information

Size	Unit Price	Same Day	2-3 Business Days	Other Lead Time	Shipping Information
1VIAL	₩253,500	7	0	Contact Us	Shipping Simulation

* Please contact our distributor of SEJIN CI Co., Ltd. if you would like to purchase TCI products. The above prices do not include freight cost, customs charge and other charges to the destination. SEJIN CI Co., Ltd. (Phone: 02-2655-2480 / email:  sales@sejinci.co.kr)
* The storage conditions are subject to change without notice.

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Product Information

• Quantity : 1.0 U per vial (10 U/mL)
• Product Form : Purified Sialidase, Liquid
• Storage buffer : 20 mM Sodium phosphate buffer, pH 7.4
• Preservative : None
• Stabilizer : None

Description

• This Sialidase is a glycoside hydrolase that cleaves the glycosidic linkages of neuraminic acids. The Sialidase acts on the α2-3, α2-6, and α2-8 glycosidic linkages located on terminal sialic acid residues composed of oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrates. This enzyme is derived from Arthrobacter ureafaciens.

Unit Definition

• One unit (U) of the Sialidase is defined as the amount required to produce 1 μmol of N-acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU (Product No. M3031) per minute at 37°C under the condition of pH 5.0.

Assay Conditions

• Reaction conditions: 50 mM Sodium acetate, pH 5.0
  The reaction mixture includes the enzyme and Neu5Ac-α-4MU in 40 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding a 4-fold volume of 0.1 M sodium carbonate buffer, pH 10.0. Quantification of released 4-MU is measured by HPLC.

Storage

• Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.
  (For long-term use, aliquot and freeze after thawing.)

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