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CAS RN: 28718-90-3 | Product Number: A2412
DAPI 2HCl [for Biochemical Research]
![DAPI 2HCl [for Biochemical Research] No-Image](/medias/A2412.jpg?context=bWFzdGVyfHJvb3R8NDMyNzR8aW1hZ2UvanBlZ3xhREZrTDJoaE5pODRPVEU1TmpZeU56SXpNVEF5TDBFeU5ERXlMbXB3Wnd8ZTIwNjk2NjdhZjZlZTQyY2M1OGY0NTM1MjUyNTU1OWU5ZDRhYzlkODc4YTczMmJkZmU4YjA0ZjY1ZGViMGI4Mg)
![DAPI 2HCl [for Biochemical Research] No-Image](/medias/Tci-440-A2412-DAPI-2HCl-was-used-to-stain-the-nuclei.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8NTQyNDJ8aW1hZ2UvanBlZ3xhREUzTDJoaVpDODVOVEUwTVRNeE16STBPVFU0TDFSamFTMDBOREJmUVRJME1USXRSRUZRU1MweVNFTnNMWGRoY3kxMWMyVmtMWFJ2TFhOMFlXbHVMWFJvWlMxdWRXTnNaV2t1YW5CbnxkM2Q0MWFlNDBjOWI2NDQ2MTk1ZDAyMTM5ZTI5NjE3Y2I0N2NhNzYzY2M4MzlmZmM0NzAyMTc0MDk4MGVhYjA3)
Purity: >90.0%(HPLC)
Synonyms:
- 4',6-Diamidino-2-phenylindole Dihydrochloride
- 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride
- 6-Amidino-2-(4-amidinophenyl)indole Dihydrochloride
Product Documents:
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* The storage conditions are subject to change without notice.
* The storage conditions are subject to change without notice.
Product Number | A2412 |
Purity / Analysis Method | >90.0%(HPLC) |
Molecular Formula / Molecular Weight | C__1__6H__1__5N__5·2HCl = 350.25 |
Physical State (20 deg.C) | Solid |
Storage Temperature | Frozen (<0°C) |
Condition to Avoid | Light Sensitive,Heat Sensitive |
CAS RN | 28718-90-3 |
Reaxys Registry Number | 4894417 |
PubChem Substance ID | 135727109 |
MDL Number | MFCD00012681 |
Specifications
Appearance | Light yellow to Yellow to Orange powder to crystal |
Purity(HPLC) | min. 90.0 area% |
DNA staining test | to pass test |
Properties (reference)
GHS
Related Laws:
Transport Information:
H.S.code* | 2933.99-000 |
Application
Fluorescence Staining of Cell Nucleus
DAPI is an organic dye which binds strongly to DNA. DAPI emits a blue fluorescence (Maximum fluorescent wavelength; 461 nm) by the irradiation of UV light (Maximum excitation wavelength; 358 nm).
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.
HeLa cells were fixed with 4% paraformaldehyde, and permerabilized with 0.2% Triton X-100.
HeLa cells nuclei were stained with 1 µg/mL DAPI [A2412] solution.
HeLa cells nuclei were stained with 1 µg/mL DAPI [A2412] solution.
References
- Labeling Nuclear DNA Using DAPI
PubMed Literature
Articles/Brochures
TCIMAIL
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