Maximum quantity allowed is 999
Please select the quantity
CAS RN: 59793-96-3 | Product Number: A1844
endo-α-N-Acetylgalactosaminidase (=Endo-α) Recombinant: from Bifidobacterium longum expressed in Escherichia coli
×
Purity:
Synonyms:
- Endo-α Recombinant: from Bifidobacterium longum expressed in Escherichia coli
Product Documents:
| Size | Unit Price | Saitama (Kawaguchi) | Hyogo (Amagasaki) | Stock in other WH |
Shipping Information
|
|---|---|---|---|---|---|
| 1VIAL |
¥19,100
|
12 | It can be shipped from Saitama warehouse on the same day* | Contact Us |
* Available Stock: Prompt shipment (for products) in Saitama/Hyogo warehouse. Stock In Other WH: Shipment in about 2-3 Business Days from Saitama warehouse. Please contact us
if you need further information.
* The displayed price is the unit price and does not include consumption tax. The unit prices displayed are the latest and are subject to change without notice.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
* The displayed price is the unit price and does not include consumption tax. The unit prices displayed are the latest and are subject to change without notice.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
- Quantity : 100 mU per vial
- Product Form : Purified Endo-α, Lyophilized powder
- Preservative : None
- Stabilizer : None
Description
- This Endo-α is a glycoside hydrolase that recognizes the structure of the Galβ1-3GalNAc disaccharide α-linked with a hydroxyl group and it releases Galβ1-3GalNAc (galacto-N-biose) by hydrolysis. The Endo-α gene is derived from Bifidobacterium longum, and its recombinant protein is expressed in Escherichia coli.
Unit Definition
- One unit (U) of the Endo-α is defined as the amount required to produce 1 μmol of Galβ1-3GalNAc from Galβ1-3GalNAc α-pNP (Product No. G0375) per minute at 37°C under the condition of pH 5.0.
Assay Conditions
- Reaction conditions: 50 mM Sodium acetate, pH 5.0
The reaction mixture includes the enzyme and Galβ1-3GalNAc α-pNP in 20 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding 30 μL of 0.25 M sodium carbonate. Quantification of released pNP is measured by a UV spectrometer.
Storage
- Store at -80°C in its lyophilized powder form. Use promptly after dissolving in buffer.
Related Products
- I1205 Sialidase (Neuraminidase)
- I1206 Sialidase (Neuraminidase) Agarose
- G0622 O-Glycosidase for core1 (Endo-α and Sialidase mix)
- Other oligosaccharide-releasing enzymes
- [Endo-type N-glycanase (ENGase)]
- A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
- E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
- G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
- [Endo-type O-glycanase]
- A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
- [Endo-type glycoceramidase]
- R0240 rEGCase I
- R0241 rEGCase I assisted by Activator II
- R0242 rEGCase II
- R0243 rEGCase II assisted by Activator II
- [Endo-type glycosaminoglycanase]
- K0069 Keratanase II (for hydrolysis of Keratan sulfate)
| Product Number | A1844 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature | Frozen (-80°C) |
| Condition to Avoid | Heat Sensitive |
| CAS RN | 59793-96-3 |
| Enzyme Commission Number | 3.2.1.97 |
| MDL Number | MFCD00131003 |
Specifications
| Appearance | White powder to crystal |
| Titer | 80 ～ 120 mUnit/1 Vial |
Properties (reference)
GHS
Related Laws:
Transport Information:
Application
Argatroban: A Direct Thrombin Inhibitor Act as an Anticoagulant
References
- K. Fujita, F. Oura, N. Nagamine, T. Katayama, J. Hiratake, K. Sakata, H. Kumagai, K. Yamamoto, J. Biol. Chem. 2005, 280, 37415.
- H. Ashida, K. Yamamoto, T. Murata, T. Usui, H. Kumagai, Arch. Biochem. Biophys. 2000, 373, 394.
- T. Katayama, K. Fujita, K. Yamamoto, J. Biosci. Bioeng. 2005, 99, 457.
Articles/Brochures
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
Specifications
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.
A sample C of A for this product is not available at this time.
Analytical Charts
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
The requested analytical chart is not available. Sorry for the inconvenience.