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CAS RN: | Product Number: C4087
Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Hyderabad | Japan* | Quantity |
|---|---|---|---|---|
| 1KIT |
₹15,500.00
|
Contact Us | 26 |
|
*Upon orders receipt, Hyderabad stocks will be dispatched on the same day.
*Items available in Japan warehouse will be dispatched in 10-12 working days.
*INR price is exclusive of domestic taxes applicable.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
- Components : Chemiluminescence HRP Substrate Solution A 100 mL×1, Chemiluminescence HRP Substrate Solution B 100 mL×1
- Volume : 1 kit (for 2000 cm2 of membrane)
Description
- This product is a reagent for the chemiluminescent detection of HRP-labeled probes that react with proteins transferred to the membrane by Western blotting. Immediately before use, solutions A and B are mixed 1:1 and applied to the membrane. It allows the detection of proteins at the nanogram level.
Storage
- Store at 4 °C, protected from light. Use within 6 months.
Direction for Use
-
- Bring Solution A and Solution B to room temperature.
- Prepare a blotting membrane covered with HRP-conjugated probe.
- Wash the membrane three times for 10 minutes each time with 1× TBS-T buffer.
- Mix Solution A and Solution B in a 1:1 ratio immediately before use. The recommended volume is 0.1 mL/cm2 membrane.
- Place the membrane with the protein blotted side up on the plastic wrap or polyethylene sheet.
- Pour the mixture over the surface of the membrane.
- Incubate the membrane with this product for 60 seconds at room temperature.
- Remove excess mixture using paper.
- Place the membrane in a new wrap or polyethylene sheet.
- Image the membrane using an imaging system or X-ray film.
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- C3384 4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]
- N1113 Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
- T3855 TMB [for Western blotting] (Ready-to-use solution)
| Product Number | C4087 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
Specifications
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H317 : May cause an allergic skin reaction. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P272 : Contaminated work clothing should not be allowed out of the workplace. P261 : Avoid breathing mist or vapors. P280 : Wear protective gloves. P302 + P352 : IF ON SKIN: Wash with plenty of water. P362 + P364 : Take off contaminated clothing and wash it before reuse. P333 + P313 : If skin irritation or rash occurs: Get medical advice/ attention. |
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