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CAS RN: | Product Number: B6279

Nervous Tissue Protein Extraction Buffer


Purity:
Synonyms:
Product Documents:
100ML
₹5,700.00
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Supplemental Product Information:

Product Information

Volume : 100 mL
Additives : WITHOUT protease inhibitors

Description

This product is supplied as a ready-to-use solution to lyse nervous tissue. Extracted protein can be used in downstream applications such as western blotting and protein quantification. This product does not include protease inhibitors.


Directions for Use

1. Wash nervous tissue twice with PBS.
2. Weigh samples. For each gram of sample, add 10 mL of Nervous Tissue Protein Extraction Buffer (Product No. B6279) and homogenize.
3. Transfer the homogenate to a new tube.
4. Incubate on ice for 10 minutes.
5. Centrifuge the sample at 10,000 × g for 10 minutes.
6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.


Storage

Store at 4 °C and use within 6 months.


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Product Number B6279
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Heat Sensitive
Specifications
Appearance Colorless to Almost colorless clear liquid
Suitability for protein-analysis to pass test
pH 7.0 to 8.0
Properties (reference)
Specific Gravity (20/20) 1.01
GHS
Related Laws:
Transport Information:
Application
Nervous Tissue Protein Extraction

References


Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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