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CAS RN: 127274-91-3 | Product Number: D6263

DiD Solution [for Cell Membrane Staining]


Purity:
Synonyms:
  • 2-[5-(3,3-Dimethyl-1-octadecylindolin-2-ylidene)penta-1,3-dien-1-yl]-3,3-dimethyl-1-octadecyl-3H-indol-1-ium Perchlorate Solution
Product Documents:
1ML
€226.00
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*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).


Supplemental Product Information:

Product Information

  • Concentration : 1 mM
  • Excitation / Emission : 657 nm / 678 nm

Description

  • DiD is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiD which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.

Storage

  • Store at room temperature and protect from light.

Directions for Use: Staining live cells

    1. Dilute DiD solution to 5 μM with culture medium.
    2. Remove medium from cells and replace with 5 μM DiD-containing medium.
    3. Incubate cells at 37 °C for 10 minutes.
    4. Remove medium and wash cells 3 times with PBS.
    5. Observe stained cell membranes via a fluorescence microscope.

Directions for Use: Staining fixed cells

    1. Remove culture medium and wash cells twice with PBS.
    2. Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
    3. Dilute DiD solution to 5 μM with PBS.
    4. Remove 4% paraformaldehyde and add 5 μM DiD-containing PBS to cells.
    5. Incubate cells at 37 °C for 10 minutes.
    6. Remove medium and wash cells 3 times with PBS.
    7. Observe stained cell membranes via a fluorescence microscope.

Product Number D6263
Molecular Formula / Molecular Weight C61H99ClN2O4 = 959.92 
Physical State (20 deg.C) Liquid
Storage Temperature Room Temperature (Recommended in a cool and dark place, <15°C)
CAS RN 127274-91-3

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