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Published TCIMAIL newest issue No.197
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Ready-to-use Solution for Protein Extraction from Nervous Tissue
No.188(November 2021)
1 is a ready-to-use solution for protein extraction from nervous tissue. By suspending tissue in 1 and then centrifuging, the supernatant containing proteins can be obtained. Extracted protein can be used in downstream applications such as electrophoresis and western blotting.
Application example
[Protein extraction]
- Wash mouse brain twice with PBS.
- Weigh samples. For each gram of sample, add 10 mL of 1 and homogenize.
- Incubate on ice for 10 minutes.
- Centrifuge the sample at 10000 × g for 10 minutes at 4 °C and collect lysate fraction.
[Protein quantification]: measurement of protein concentration in lysate fraction by pyrogallol red method
[Electrophoresis / Western blotting]: Detection of specific protein in lysate fraction
Synaptophysin (38 kDa) in synaptic vesicle can be extracted from mouse brain efficiently.
References
- 1) Quantification of the Brain Proteome in Alzheimer's Disease Using Multiplexed Mass Spectrometry
- 2) Optimized protein extraction from cryopreserved brain tissue samples.
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