text.skipToContent text.skipToNavigation

TCI uses cookies to personalize and improve your user experience. By continuing on our website, you accept the use of cookies. You can change or update your cookiesettings at any time.

Maximum quantity allowed is 999

Please select the quantity

CAS RN: | Product Number: T3854

TMB [for ELISA] (Ready-to-use solution)

× TMB [for ELISA] (Ready-to-use solution)

Purity:
Synonyms:
  • 3,3',5,5'-Tetramethylbenzidine [Soluble chromogenic substrate for ELISA] (Ready-to-use solution)
Product Documents:
SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications

Supplemental Product Information
100ML
€62.00
 1   30  

*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).

Supplemental Product Information:

Product Information
Volume : 100 mL

Description
This product is supplied as a ready-to-use solution containing 3,3',5,5'-Tetramethyl -benzidine (TMB) and hydrogen peroxide for ELISA. When this product reacts with horseradish peroxidase (HRP), a blue colored soluble reaction product appears. Its absorbance can be measured at 650 nm. After terminating the reaction by adding hydrochloric acid, the reaction product becomes yellow and its absorbance can be measured at 450 nm.

Direction for Use
The following steps are for a 96-well plate format.
1. Bring TMB solution (Product No. T3854) to room temperature.
2. Gently mix the TMB solution (Product No. T3854).
3. Add 100uL of TMB solution (Product No. T3854) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. To terminate the reaction, add 100uL of 1 N HCl (Product No. H1202) to each well.
6. Within 1 hour of terminating the reaction, measure the absorbance of each well at 450 nm.

Storage
The product should be stored at 4 ºC, and used within 6 months.
Precipitations sometimes arise, but they do not affect the quality.

Related Products
T3855 TMB (Ready-to-use solution) [for Western blotting]
T2573 3,3',5,5'-Tetramethylbenzidine [for Biochemical Research]
H1202 Hydrochloric Acid (1mol/L)
H1621 Horseradish Peroxidase Maleimide Conjugate
A3075 Anti-6xHis Monoclonal Antibody (6A12) HRP Conjugate
A3187 Anti-Protein A Chicken Polyclonal Antibody HRP Conjugate
A3195 Anti-α Gal Chicken Polyclonal Antibody HRP Conjugate
G0407 Goat Anti-Mouse IgG HRP Conjugate
G0417 Goat Anti-Mouse IgM HRP Conjugate
G0418 Goat Anti-Rabbit IgG HRP Conjugate
P2466 Protein A HRP Conjugate
S0972 Streptavidin HRP Conjugate
S0999 Sheep Anti-Chicken IgY HRP Conjugate

Product Number T3854
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Heat Sensitive
Specifications
Appearance Colorless to Almost colorlessclear liquid
Peroxidase detection test to pass test
Properties (reference)
GHS
Pictogram Pictogram Pictogram
Signal Word Danger
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
H372 : Causes damage to organs through prolonged or repeated exposure.
H370 : Causes damage to organs.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P260 : Do not breathe mist or vapours.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor.
Related Laws:
Transport Information:
HS Number 3822190090
Application
TMB [for ELISA] (Ready-to-use solution)

References

  • Cold Spring Harbor Laboratory Press, New York
    • E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p404, p506, p594


Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.

The requested SDS is not available.

Please Contact Us for more information.
Specifications
C of A & Other Certificates
Please enter Lot Number Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.

The product with the lot number searched for has been discontinued and no related documentation is available.
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.

A sample C of A for this product is not available at this time.

Analytical Charts
Please enter Lot Number Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.

The requested analytical chart is not available. Sorry for the inconvenience.

The product with the lot number searched for has been discontinued and no related documentation is available.
Other Documents

Related Products

Session Status
Your session will timeout in 10 minutes. You will be redirected to the HOME page after session timeout. Please click the button to continue session from the same page. minute. You will be redirected to the HOME page after session timeout. Please click the button to continue session from the same page.
Continue Operation
Your session has timed out. You will be redirected to the HOME page.
Close