DiD Solution [for Cell Membrane Staining]

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Synonyms:

• 2-[5-(3,3-Dimethyl-1-octadecylindolin-2-ylidene)penta-1,3-dien-1-yl]-3,3-dimethyl-1-octadecyl-3H-indol-1-ium Perchlorate Solution

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Supplemental Product Information

Size	Unit Price	Belgium	Japan*	Quantity
1ML	€226.00	Contact Us	14

*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).

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Product Information

• Concentration : 1 mM
• Excitation / Emission : 657 nm / 678 nm

Description

• DiD is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiD which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.

Storage

• Store at room temperature and protect from light.

Directions for Use: Staining live cells

• 1. Dilute DiD solution to 5 μM with culture medium.
  2. Remove medium from cells and replace with 5 μM DiD-containing medium.
  3. Incubate cells at 37 °C for 10 minutes.
  4. Remove medium and wash cells 3 times with PBS.
  5. Observe stained cell membranes via a fluorescence microscope.

Directions for Use: Staining fixed cells

• 1. Remove culture medium and wash cells twice with PBS.
  2. Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
  3. Dilute DiD solution to 5 μM with PBS.
  4. Remove 4% paraformaldehyde and add 5 μM DiD-containing PBS to cells.
  5. Incubate cells at 37 °C for 10 minutes.
  6. Remove medium and wash cells 3 times with PBS.
  7. Observe stained cell membranes via a fluorescence microscope.