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Published TCIMAIL newest issue No.197
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Fluorescence/Luminescence Spectroscopy Reagents
Fluorescent dyes in bioscience research are actively utilized as they provide higher sensitivity for visualizing biomolecules such as proteins and DNA. The dyes are classified according to their structure: fluoresceins, rhodamines, coumarins, pyrenes, cyanines, etc. This section includes precursors of fluorescent dyes which are converted in vivo to fluorescence-emitting compounds (e.g. fluorescein diacetate) and which emit fluorescence after conjugation with specific molecules (e.g. ethidium bromide).
References
- Review: G. T. Hermanson, in Bioconjugate Techniques, 2nd ed., Academic Press, New York, 2008.
- R. P. Haugland, in Handbook of Fluorescent Probes and Research Products, 9th ed., ed.by J. Gregory, Molecular Probes, Inc. Eugene, 2002.
- T. Terai, T. Nagano, Curr. Opin. Chem. Biol. 2008, 12, 515.
![[[(3,5-Dimethyl-1H-pyrrol-2-yl)(3,5-dimethyl-2H-pyrrol-2-ylidene)methyl]methane](difluoroborane) (purified by sublimation)](/medias/D5724.jpg?context=bWFzdGVyfHJvb3R8NDU3MzR8aW1hZ2UvanBlZ3xhREZtTDJnNFppODRPVE13TkRRNU5qSXhNREl5TDBRMU56STBMbXB3Wnd8ZDdiOWZmYjNlZWUyNmZkMDEwNzM3MDMxNTQ0Y2QxM2M4YWRmNjAwZTBmMDI1Yzk1M2VjM2U1ZjA2YTc1YTY4Nw "[[(3,5-Dimethyl-1H-pyrrol-2-yl)(3,5-dimethyl-2H-pyrrol-2-ylidene)methyl]methane](difluoroborane) (purified by sublimation)")
| Product Number | D5724 |
| CAS RN | 121207-31-6 |
| Purity / Analysis Method: | >99.5%(GC) |
| Product Number | D5730 |
| CAS RN | 91-44-1 |
| Purity / Analysis Method: | >99.5%(GC)(T) |
![2,3-Naphthalenedialdehyde [Fluorimetric Reagent for Primary Amines]](/medias/A5594.jpg?context=bWFzdGVyfHJvb3R8Mzc1MjF8aW1hZ2UvanBlZ3xhR0UyTDJneU1DODRPVEk0TlRFNE56azVNemt3TDBFMU5UazBMbXB3Wnd8MTdmZjdmNDQ4Y2NmNzRmMzIwNWI5ZDI2MzE2ZjM2YTM2YWI5NjZmMGNmY2NkM2MzYWMwMTM2MzlhOTYwODRjNg "2,3-Naphthalenedialdehyde [Fluorimetric Reagent for Primary Amines]")
| Product Number | A5594 |
| CAS RN | 7149-49-7 |
| Purity / Analysis Method: | >99.0%(GC) |
| Product Number | C3640 |
| CAS RN | 53518-18-6 |
| Purity / Analysis Method: | >99.0%(T)(HPLC) |
| Product Number | D5727 |
| CAS RN | 87-01-4 |
| Purity / Analysis Method: | >99.0%(GC)(T) |
![D-(-)-Luciferin [Chemiluminescence Reagent]](/medias/A5030.jpg?context=bWFzdGVyfHJvb3R8Mjg4MzB8aW1hZ2UvanBlZ3xhRGN6TDJnNVl5ODRPVEk0TlRFeU56QTBOVFF5TDBFMU1ETXdMbXB3Wnd8NTY5ZjI1MmY0YmRjZTEzNjg1NzM5M2Y0YjVmNTAxNmY1MTliNTNjNmIzYTczYWFlNGFhNDhkNDYyMjU4MjJmMQ "D-(-)-Luciferin [Chemiluminescence Reagent]")
| Product Number | A5030 |
| CAS RN | 2591-17-5 |
| Purity / Analysis Method: | >98.0%(HPLC) |
![Rhodamine B [Ion association reagent for photometric and fluorimetric analysis]](/medias/A5102.jpg?context=bWFzdGVyfHJvb3R8MzU3ODB8aW1hZ2UvanBlZ3xhRGRpTDJnME15ODRPVEk0TlRFek1qSTRPRE13TDBFMU1UQXlMbXB3Wnd8YzRjOWM1OTVjYWI5Y2FhZjkzYWJmNjM5YTNkYjk3YzRhOTcyYTc2Y2I3ZWUyN2E5ZDBmZTRkMzg1ZDk5ZGY1ZQ "Rhodamine B [Ion association reagent for photometric and fluorimetric analysis]")
| Product Number | A5102 |
| CAS RN | 81-88-9 |
| Purity / Analysis Method: | >98.0%(T) |
![Isoluminol [Chemiluminescence reagent]](/medias/A5300.jpg?context=bWFzdGVyfHJvb3R8MzQ4Nzh8aW1hZ2UvanBlZ3xhREpsTDJneU5pODRPVEk0TlRFME1qRXhPRGN3TDBFMU16QXdMbXB3Wnd8ZDU2ZDFkY2NlNzc5ZWU3NTZmNDI0ODE2M2M0MDc1MTcxNzk5MGMzMjBmOWIxNjI5Yjk2ZmM0NzhjMjI0ZmY2Zg "Isoluminol [Chemiluminescence reagent]")
| Product Number | A5300 |
| CAS RN | 3682-14-2 |
| Purity / Analysis Method: | >98.0%(T)(HPLC) |
![Bis(2,4,6-trichlorophenyl) Oxalate [Chemiluminescence reagent for the determination of fluorescent compounds]](/medias/A5302.jpg?context=bWFzdGVyfHJvb3R8MzAwNDV8aW1hZ2UvanBlZ3xhR1ZsTDJneU9TODRPVEk0TlRFME16UXlPVFF5TDBFMU16QXlMbXB3Wnd8ZTgyOGY4ZjI3ZmM1YWY0MzA5YTc4MTk1NjFjYWNmMGI2MGIyYjc2YzBmYzdlMTYzMGQ3NmI0MDdiMmFiZmQxNw "Bis(2,4,6-trichlorophenyl) Oxalate [Chemiluminescence reagent for the determination of fluorescent compounds]")
| Product Number | A5302 |
| CAS RN | 1165-91-9 |
| Purity / Analysis Method: | >98.0%(GC)(T) |
![Bis(2,4-dinitrophenyl) Oxalate [Chemiluminescence reagent for the determination of fluorescent compounds by HPLC and FIA]](/medias/A5303.jpg?context=bWFzdGVyfHJvb3R8Mjg1MTN8aW1hZ2UvanBlZ3xhR1ZqTDJneVl5ODRPVEk0TlRFME5EQTRORGM0TDBFMU16QXpMbXB3Wnd8M2Q2MzNhYmM3ZTMxZGYyYzQ2MzI0ZTJjM2VhZjBlNzEyZWQ4YzA1ZjJhNWU0NDI1OWVkMWI0ZjI1MDQxZjE0Yw "Bis(2,4-dinitrophenyl) Oxalate [Chemiluminescence reagent for the determination of fluorescent compounds by HPLC and FIA]")
| Product Number | A5303 |
| CAS RN | 16536-30-4 |
| Purity / Analysis Method: | >98.0%(T) |
![CLA [Chemiluminescence Reagent]](/medias/A5307.jpg?context=bWFzdGVyfHJvb3R8MzM2NzR8aW1hZ2UvanBlZ3xhR0pqTDJnek15ODRPVEk0TlRFME5qQTFNRGcyTDBFMU16QTNMbXB3Wnd8ZmZjMmE3YzUxMDE0NWQzOGM3ODFkYjU3NWU4OTM2MDYyNGIxNTgyYjAxOTczZTBmMTQ2YzE4N2QwMDgwNDc2YQ "CLA [Chemiluminescence Reagent]")
| Product Number | A5307 |
| CAS RN | 19953-58-3 |
| Purity / Analysis Method: | >98.0%(HPLC) |
![MCLA [Chemiluminescence Reagent]](/medias/A5309.jpg?context=bWFzdGVyfHJvb3R8MjkzODh8aW1hZ2UvanBlZ3xhRGRrTDJnek5DODRPVEk0TlRFME5qY3dOakl5TDBFMU16QTVMbXB3Wnd8MGJmMzQ4ZDk0ZWIyOWJkNzFjNmU5ZWJlMDJjYTg4ZWUwYjY5Mjc1ZGViZWFhN2MyNjIwZDRlYjJjNzAyZmM4OQ "MCLA [Chemiluminescence Reagent]")
| Product Number | A5309 |
| CAS RN | 128322-44-1 |
| Purity / Analysis Method: | >98.0%(HPLC) |
Product Number: D5724 |
Purity / Analysis Method:
>99.5%(GC)
Product Number: D5730 |
Purity / Analysis Method:
>99.5%(GC)(T)
Product Number: A5594 |
Purity / Analysis Method:
>99.0%(GC)
Product Number: C3640 |
Purity / Analysis Method:
>99.0%(T)(HPLC)
Product Number: D5727 |
Purity / Analysis Method:
>99.0%(GC)(T)
Product Number: A5030 |
Purity / Analysis Method:
>98.0%(HPLC)
Product Number: A5102 |
Purity / Analysis Method:
>98.0%(T)
Product Number: A5300 |
Purity / Analysis Method:
>98.0%(T)(HPLC)
Product Number: A5302 |
Purity / Analysis Method:
>98.0%(GC)(T)
Product Number: A5303 |
Purity / Analysis Method:
>98.0%(T)
Product Number: A5307 |
Purity / Analysis Method:
>98.0%(HPLC)
Product Number: A5309 |
Purity / Analysis Method:
>98.0%(HPLC)
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