Maximum quantity allowed is 999
CAS RN: | Product Number: Y0021
E.coli / Yeast Protein Extraction Buffer
Purity:
Size | Unit Price | Same Day | 2-3 Business Days |
---|---|---|---|
100ML |
$91.00
|
4 | 2 |
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TCI
to order our products. The above prices do not include freight cost, customs, and other charges to the destination.
* The storage conditions are subject to change without notice.
Supplemental Product Information:
Product Information
Volume : 100 mLAdditives : WITHOUT protease inhibitors
Description
This product is supplied as a ready-to-use solution to lyse cultured E.coli / yeast cells. Extracted protein can be used in downstream applications such as Western blotting and protein quantification. This product does not include protease inhibitors.
Directions for Use: Lysis of E.coli cells
1. Centrifuge E.coli cells at 5000 × g for 10 minutes.
2. Remove as much medium as possible from pellet.
3. Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
4. Incubate for 10 minutes at room temperature.
5. Centrifuge lysate at 15,000 × g for 10 minutes.
6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Directions for Use: Lysis of yeast cells
1. Centrifuge yeast cells at 3000 × g for 10 minutes.
2. Remove as much medium as possible from pellet.
3. Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
4. Agitate at room temperature for 10 minutes.
5. Centrifuge lysate at 14,000 × g for 10 minutes.
6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Storage
Store at 4 °C and use within 6 months.
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Product Number | Y0021 |
Physical State (20 deg.C) | Liquid |
Storage Temperature | Refrigerated (0-10°C) |
Condition to Avoid | Heat Sensitive |
Appearance | Colorless to Almost colorlessclear liquid |
Suitability for protein-analysis (E.coli) | to pass test |
Suitability for protein-analysis (Yeast) | to pass test |
Specific Gravity (20/20) | 1.06 |
H.S.code* | 3822.19-000 |
References
- Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
Articles/Brochures
[TCIMAIL No.188] Ready-to-Use Solution for Protein Extraction from E. coli / Yeast Cells
Safety Data Sheet (SDS)
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Specifications
C of A & Other Certificates
Sample C of A
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Analytical Charts
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