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CAS RN: | Product Number: Y0021

E.coli / Yeast Protein Extraction Buffer


Purity:
Synonyms:
Product Documents:
100ML
$91.00
4   2  

* Please contact our distributors or TCI to order our products. The above prices do not include freight cost, customs, and other charges to the destination.
* The storage conditions are subject to change without notice.


Supplemental Product Information:

Product Information

Volume : 100 mL
Additives : WITHOUT protease inhibitors

Description

This product is supplied as a ready-to-use solution to lyse cultured E.coli / yeast cells. Extracted protein can be used in downstream applications such as Western blotting and protein quantification. This product does not include protease inhibitors.


Directions for Use: Lysis of E.coli cells

1. Centrifuge E.coli cells at 5000 × g for 10 minutes.
2. Remove as much medium as possible from pellet.
3. Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
4. Incubate for 10 minutes at room temperature.
5. Centrifuge lysate at 15,000 × g for 10 minutes.
6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.


Directions for Use: Lysis of yeast cells

1. Centrifuge yeast cells at 3000 × g for 10 minutes.
2. Remove as much medium as possible from pellet.
3. Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
4. Agitate at room temperature for 10 minutes.
5. Centrifuge lysate at 14,000 × g for 10 minutes.
6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.


Storage

Store at 4 °C and use within 6 months.


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Product Number Y0021
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Heat Sensitive
Specifications
Appearance Colorless to Almost colorlessclear liquid
Suitability for protein-analysis (E.coli) to pass test
Suitability for protein-analysis (Yeast) to pass test
Properties (reference)
Specific Gravity (20/20) 1.06
GHS
Related Laws:
Transport Information:
H.S.code* 3822.19-000
*This code is applied to the products when TCI exports from Japan and not for import in your country.
Application
E.coli / Yeast Protein Extraction

References

Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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