Published TCIMAIL newest issue No.197
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Gel Negative-Staining Kit for Rapid and Highly Sensitive Protein Detection
Detecting protein bands in a polyacrylamide gel after electrophoresis requires the gel to be stained. Common staining methods include Coomassie Brilliant Blue staining, silver staining, fluorescence staining, and negative staining. Negative staining is a method in which only regions of gel containing protein remain unstained, while the remainder of the gel is stained white.1) Bands can be visualized by placing the gel against a dark background.
Gel Negative Stain kit (1) can be used to detect protein bands with high sensitivity in as little as 20 minutes. Furthermore, stained gels can easily be destained using the included destaining solution, with the destained gel is able to be used in further downstream applications, such as Western blotting and mass spectrometry.2,3)
Figure 1. Gels containing molecular weight markers at various concentrations were stained with 1.
Figure 2. A: Gels loaded with mouse serum and stained with 1. B: The gel in Figure 2A was destained and mouse IgG was detected via Western Blotting.
Related Products
- G0615
- Gel Negative Stain kit [for Electrophoresis]
- A3217
- 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
- A3218
- 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
- B5834
- 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
- B6104
- 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
- B6105
- 6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
- B6110
- 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
- C3488
- Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
References
- 1) Understanding the mechanism of the zinc-ion stains of biomacromolecules in electrophoresis gels: Generalization of the reverse-staining technique
- 2) A procedure for protein elution from reverse-stained polyarcylamide gels applicable at the low picomole level: An alternative route to the preparation of low abundance proteins for microanalysis
- 3) High Yield Elution of Proteins from Sodium Dodecyl Sulfate–Polyacrylamide Gels at the Low-Picomole Level. Application to N-Terminal Sequencing of a Scarce Protein and to In-Solution Biological Activity Analysis of On-Gel Renatured Proteins
