用于合成识别DNA序列的PI聚酰胺的原料

PIPs(吡咯-咪唑 (Py-Im)聚酰胺)中的氨基侧和羰基侧反向平行相对，形成Py/Py或Py/Im对。它们通过氢键识别特定的DNA碱基对，进入DNA的双螺旋结构的小沟，选择性地与碱基序列结合。通过改变Py和Im的序列，可以设计和结合任意DNA碱基序列^1-4)。具有Boc或Fmoc保护的氨基的1-甲基-吡咯 (1, 2)和1-甲基-咪唑 (3, 4)单体已经被用作合成PIPs的原料。此外，难以通过固相合成延长的Pylm砌块 (5)也能被利用^5-7)。

关键词

B6351

4-(Boc-氨基)-1-甲基-1H-吡咯-2-甲酸 (= Boc-Py-OH)

F1314

4-(Fmoc-氨基)-1-甲基-1H-吡咯-2-甲酸 (= Fmoc-Py-OH)

B6350

4-(Boc-氨基)-1-甲基-1H-咪唑-2-甲酸 (= Boc-Im-OH)

F1313

4-(Fmoc-氨基)-1-甲基-1H-咪唑-2-甲酸 (= Fmoc-Im-OH)

F1315

Fmoc-PyIm-COOH

B4291

N-Boc-4-氨基丁酸

F0911

N-Fmoc-4-氨基丁酸

文献

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