Fluorescent dyes in bioscience research are actively utilized as they provide higher sensitivity for visualizing biomolecules such as proteins and DNA. The dyes are classified according to their structure: fluoresceins, rhodamines, coumarins, pyrenes, cyanines, etc. This section includes precursors of fluorescent dyes which are converted in vivo to fluorescence-emitting compounds (e.g. fluorescein diacetate) and which emit fluorescence after conjugation with specific molecules (e.g. ethidium bromide).
References
- Review: G. T. Hermanson, in Bioconjugate Techniques, 2nd ed., Academic Press, New York, 2008.
- R. P. Haugland, in Handbook of Fluorescent Probes and Research Products, 9th ed., ed.by J. Gregory, Molecular Probes, Inc. Eugene, 2002.
- T. Terai, T. Nagano, Curr. Opin. Chem. Biol. 2008, 12, 515.
