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Anti-Non-human Glycoform Antibodies for Flow Cytometry
No.195(March 2024)
Non-human glycoforms are known to trigger immune reactions in humans.1,2) At TCI, we have developed a plethora of chicken polyclonal antobodies against such glycoforms, including the αGal epitope (Galα1-3Gal) and N-glycolylneuraminic acid (Neu5Gc), in order to aid in their rapid, detection. 1 and 2 are anti-non-human glycoform antibodies conjugated with R-Phycoerythrin (R-PE) for use in flow cytometry, enabling the specific detection of such non-human glycoforms on the cell surface.
Figure. Detection of non-human glycoforms in porcine peripheral blood granulocytes
Peripheral blood cells were collected from miniature pig blood. 1, 2 and an isotype control were prepared at a concentration of 0.01 mg/mL and incubated with cells for 20 min at 4 °C. Subsequent analysis of samples was performed on a flow cytometer.
References
- 1) Delayed anaphylaxis, angioedema, or urticaria after consumption of red meat in patients with IgE antibodies specific for galactose-α-1,3-galactose
- 2) At least two Fc Neu5Gc residues of monoclonal antibodies are required for binding to anti-Neu5Gc antibody
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