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Guaranteed Analytical Reagents for Oligonucleotides
No.192(March 2023)
Triethylammonium acetate aqueous solution and triethylamine/1,1,1,3,3,3-hexafluoro-2-propanol (= HFIP) aqueous solution are broadly utilized as the moving phase for the purification/analysis of oligonucleotides.1) Especially, triethylamine-HFIP buffer improves ESI-MS sensitivity in the detection of oligonucleotide LC-MS analysis.2) TCI lines up triethylammonium acetate aqueous solution (1), triethylamine (2), and HFIP (3), which are guaranteed the UV absorptions in the analytical range of oligonucleotides like in the below table. Suitable buffer solutions can be easily prepared by diluting 1 or mixing/diluting the required amounts of 2 and 3.
References
- 1) Separation of small DNA and RNA oligonucleotides by high-performance anion-exchange liquid chromatography
- 2) Analysis of oligonucleotides by HPLC-electrospray ionization mass spectrometry
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