Clearing Reagent iCBiofilm-H2 [for Biofilm]

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SDS | Spécifications | CoA et autres Certificats | Graphiques analytiques

Informations générales | Spécifications et Propriétés | Sécurité et Réglementaire | Applications & Litterature

Informations supplémentaires sur le produit

Taille	Prix ​​unitaire	Philadelphia, PA	Portland, OR	Japon *	Quantité
8ML	$99.00	Contactez-nous	Contactez-nous	9

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*Les articles en stock localement sont expédiés dans un délai de 1 à 2 jours. Les articles en stock au Japon peuvent être expédiés depuis un entrepôt aux Etats-Unis dans un délai de 2 semaines. Veuillez contacter TCI pour connaître les délais de livraison des articles qui ne sont pas en stock. Cela ne concerne par les articles réglementés et les articles expédiés sur carboglace.

Product Information

• Size : 8 mL

Description

• Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms.
  In order to observe the structures of biofilms with a thickness of more than several tens of micrometers, Clearing Reagent iCBiofilm-H2 has been developed. iCBioflim-H2 is supplied as a stock solution. Before adding to biofilm, iCBiofilm-H2 has to be diluted with deionized water to appropriate concentration (undiluted iCBiofilm-H2 may work well). By adding iCBiofilm-H2 solution to a biofilm, the biofilm becomes transparent within several seconds and easy-to-observe by light microscopy such as confocal laser scanning microscopy.
  *This product was developed by Associate Prof. Sugimoto, the Jikei University School of Medicine.

Storage

• Stored at room temperature and used within 6 months.

Direction for Use

• 1. Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
  2. After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
  3. Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
  4. Stain using appropriate fluorescence reagents.
  5. Dilute iCBiofilm-H2 (Product No. T4032) with deionized water to an appropriate concentration.
     Recommendation ratio; iCBiofilm-H2 : water = 2 : 1 or 1 : 1
     If you need the best performance of iCBioflim-H2 for a target biofilm, please consider to examine different concentrations of iCBiofilm-H2.
  6. Remove staining reagents and add diluted or undiluted iCBiofilm-H2 solution (about 500 µL – 1 mL).
  7. Observe the biofilm by optical microscopy.

Related Products

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• I1275 Clearing Reagent iCBiofilm-D [for Biofilm]