Tissue-Clearing Reagent iTOMEI-D [for Plants]

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Synonymes

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SDS | Spécifications | CoA et autres Certificats | Graphiques analytiques

Informations générales | Spécifications et Propriétés | Sécurité et Réglementaire | Applications & Litterature

Informations supplémentaires sur le produit

Taille	Prix ​​unitaire	Philadelphia, PA	Portland, OR	Japon *	Quantité
5ML	$29.00	1	Contactez-nous	12
25ML	$86.00	2	1	≥60

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*Les articles en stock localement sont expédiés dans un délai de 1 à 2 jours. Les articles en stock au Japon peuvent être expédiés depuis un entrepôt aux Etats-Unis dans un délai de 2 semaines. Veuillez contacter TCI pour connaître les délais de livraison des articles qui ne sont pas en stock. Cela ne concerne par les articles réglementés et les articles expédiés sur carboglace.

"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product is a decoloring reagent for iTOMEI.

Please see a brochure (PDF) of how to use.

Description

This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene.
The mounting solution is also required to complete the iTOMEI method.
Please check directions for use for necessary reagents.

Storage

The product should be stored at 4 °C.

Directions for Use

Required reagents;

• Paraformaldehyde (PFA)
• PBS
• Stains* (option)
• Mounting solution**

==Process outline (All operations are performed at Room temperature)===============

• Tissue fixation
  Reagent : 1% PFA in PBS
  Time : 1hr
• PBS Wash ×3
• Decoloring
  Reagent : iTOMEI-D (TCI Product No. T3940)
  Time : 24hr
• PBS Wash ×3
• (Optional process Staining*, PBS Wash ×3)
• Clearing (RI matching)
  Reagent : Mounting solution**
  Time : Operation time is determined by the mounting solution.
• Mounting
  Reagent : Mounting solution**

* The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.

** Mounting solution : Please use the suitable mounting solution for objective lens.

Related Products

P0018　Paraformaldehyde
A2412　DAPI·2HCl
E0197　Evans Blue
A0132　Acridine Orange
A0321　Tris-Base
G0316　Glycerol [for Electrophoresis]
S0111　D-(+)-Sucrose
T3530　Tissue-Clearing Reagent TOMEI [for Plants]