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CAS RN: | Numéro de produit: A3331
AOL-LecBeads [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]
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Pureté:
Synonymes
- AOL-Agarose
- Aspergillus oryzae lectin (= AOL)-Agarose
Documents de produit:
| Taille | Prix unitaire | Philadelphia, PA | Portland, OR | Japon * | Quantité |
|---|---|---|---|---|---|
| 1VIAL |
$631.00
|
Contactez-nous | Contactez-nous | 13 |
|
*Les articles en stock localement sont expédiés dans un délai de 1 à 2 jours. Les articles en stock au Japon peuvent être expédiés depuis un entrepôt aux Etats-Unis dans un délai de 2 semaines. Veuillez contacter TCI pour connaître les délais de livraison des articles qui ne sont pas en stock. Cela ne concerne par les articles réglementés et les articles expédiés sur carboglace.
Informations supplémentaires sur le produit:
Product Information
Product code: A3331
Product name: AOL-LecBeads [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]
Preparation: AOL is covalently coupled with agarose gel
Quantity: 0.5 ml gel/1 ml (1:1, v/v slurry) suspended in PBS, pH6.5
Protein concentration: about 15 mg/ml gel
Binding capacity: >5 mg of glycoprotein (human Lactoferrin) /ml gel
Preservative: 0.02% [w/v] Sodium azide
Stabilizer: None
Description
The AOL is an Aspergillus oryzae recombinant of carbohydrate-binding protein (Lectin) derived from Aspergillus oryzae (The AOL gene is derived from the same origin as the host cell). This lectin preferably binds to [Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)] glycan epitopes.
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This AOL-LecBeads (0.5 ml gel) shows a binding capacity of about 2.5 mg or more human Lactoferrin [hLF] through its binding to a terminal [Fucα(1-6)/α(1-3)] glycan epitopes on hLF.
Storage
Store at 2-10°C. (Avoid freezing).
Related Products
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5 mg/ml, PBS pH6.5)
A2659 AOL-Biotin Conjugate
R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0236 rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc]
R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0239 rGRFT-LecBeads [for Manα(1-2)Man]
| Numéro de produit | A3331 |
| Etat physique (20 ° C) | Liquid |
| Condition de stockage | Refrigerated (0-10°C) |
| Condition à éviter | Heat Sensitive |
Spécifications
| Appearance | White to Almost white clear liquid to cloudy liquid |
| Concentration(Lowry method) | min. 13.0 mg/mL gel |
| Binding capacity | to pass test(min. 5 mg/mL gel, Human Lactoferrin) |
Propriétés
SGH
Lois connexes:
Informations de transport:
| Numéro du SH (importation) (TCI-A) | 3504.00.5000 |
Application
The lectin-catch procedure
< Preparation >
Buffers
Binding buffer : PBS pH7.5
Elution buffer : PBS pH7.5 containing 200 mM L-fucose [Product code: F0065] and 0.1% triton-X100 [Product code: P1775]
< Method of the lectin-catch > ※Centrifuge method
Equilibration
- Prepare the LecBeads in a new tube.
- Add the Binding buffer into the tube to equilibrate the gel.
- Centrifuge the tube at 800 ×g for 3 min and discard the supernatant. (If necessary, repeat equilibration with the Binding buffer >10-fold volume of the LecBeads appropriately.)
Binding
- Concentrate or dilute the glycoprotein sample to moderate concentration (e.g., 1 mg/ml) with the Binding buffer [Input fraction], and apply the prepared sample to the tube in which the LecBeads was preliminary equilibrated.
- Agitate the tube at 4°C (※Generally, a dissociation constants (Kd value) of lectins are tend to be decreased at lower temperature) for a few hours (or overnight).
- Centrifuge the tube at 800 ×g for 3 min and remove the supernatant [Through fraction].
- Wash the LecBeads by 5 (to 10)-fold of the slurry volume with the Binding buffer several times.
Elution
- Elute the glycoprotein from the LecBeads by agitation with an appropriate volume of the Elution buffer (e.g. same volume of the LecBeads).
- Agitate the reaction tube at 4°C or R.T. for a few hours (or overnight).
- Centrifuge the tube at 800 ×g for 3 min and remove the supernatant [Elution fraction].
- Check the resulting fractions ([Input fraction] [Through fraction] [Elution fraction]) by protein assay and SDS-PAGE. (In some cases, the repetitive elution step with a small volume of the Elution buffer might improve the elution effect. If necessary, repeat elution steps.)
- The competitive oligosaccharide included in the obtained elution fractions can be removed by ultrafiltration or dialysis using a sufficient amount of buffer.
References
- Glyco-catch method: A lectin affinity technique for glycoproteomics
- Separation technologies for glycomics
Articles / Brochures
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