DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]

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Zuiverheid:

Synoniemen

• 4',6-Diamidino-2-phenylindole Dihydrochloride (0.2mL×5) (1mg/mL in Water)
• 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride (0.2mL×5) (1mg/mL in Water)
• 6-Amidino-2-(4-amidinophenyl)indole Dihydrochloride (0.2mL×5) (1mg/mL in Water)

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Afmeting	Eenheidsprijs	België	Japan*	Hoeveelheid	Verzendinformatie
1SET	€39.00	3	29		Simulatie van verzending

*Voorraad beschikbaar in België: Verzending dezelfde dag
*Voorraad beschikbaar in Japan: Raadpleeg de verzendsimulatie voor geschatte levertijden. (met uitzondering van gereguleerde producten en zendingen met droogijs)

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Product Information

• Volume : 0.2 mL x 5 vial
• Concentration : 1.0 mg/mL

Description

• The DAPI is a fluorescent stain for labeling DNA.
  DNA-bound DAPI is readily excited with UV light and emits fluorescence in the blue region of the visible light spectrum (Excitation : 358 nm / Emission : 461 nm).

Description for Use

• Fluorescence Staining of Cell Nucleus
  1. Fix and permeabilize cells as desired.
  2. Dilute DAPI solution 1/1000 (*) with PBS or an appropriate buffer.
     *Typical working concentration used in most applications is 0.1 μg/mL - 10 μg/mL.
  3. Incubate with cells at room temperature for 5-20 minutes (**).
     **Extend Incubate time as desired.
  4. Mount sample with an appropriate medium and proceed to imaging.

Storage

• Store at -20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

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