Published TCIMAIL newest issue No.200
Maximum quantity allowed is 999
CAS RN: | Numéro de produit: Y0021
E.coli / Yeast Protein Extraction Buffer
Pureté:
*Stock disponible en Belgique : Expédition le jour même
*Stock disponible au Japon : Veuillez consulter la simulation d'expédition pour une estimation des délais d'expédition (hors articles réglementés et expédition avec Carboglace)
Informations supplémentaires sur le produit:
Product Information
- Volume : 100 mL
- Additives : WITHOUT protease inhibitors
Description
- This product is supplied as a ready-to-use solution to lyse cultured E. coli / yeast cells. Extracted protein can be used in downstream applications such as Western blotting and protein quantification. This product does not include protease inhibitors.
Storage
- Store at 4 °C.
Directions for Use: Lysis of E. coli cells
-
- Centrifuge E. coli cells at 5000 × g for 10 minutes.
- Remove as much medium as possible from pellet.
- Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 5-10 mL buffer per gram pellet.
- Incubate for 10 minutes at room temperature.
- Centrifuge lysate at 15,000 × g for 10 minutes.
- Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Directions for Use: Lysis of yeast cells
-
- Centrifuge yeast cells at 3000 × g for 10 minutes.
- Remove as much medium as possible from pellet.
- Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
- Agitate at room temperature for 10 minutes.
- Centrifuge lysate at 14,000 × g for 10 minutes.
- Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
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| Numéro de produit | Y0021 |
| Etat physique (20 ° C) | Liquid |
Condition de stockage
|
Refrigerated (0-10°C) |
| Condition à éviter | Heat Sensitive |
| Appearance | Colorless to Almost colorlessclear liquid |
| Suitability for protein-analysis (E.coli) | to pass test |
| Suitability for protein-analysis (Yeast) | to pass test |
| Densité (20/20) | 1.06 |
| N ° SH (import / export) (TCI-E) | 3822190090 |
References
- Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
Documents
[TCIMAIL No.188] Ready-to-Use Solution for Protein Extraction from E. coli / Yeast Cells
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