Imidazolium Salts as Coupling Activators for Oligonucleotide Synthesis

In oligonucleotide synthesis, condensation activators are used for coupling reactions of phosphoramidite reagents. TCI offers imidazolium salt type activators (1-5) as well as tetrazole type activators. Imidazolium salt activators are less acidic than tetrazole type activators, but they are known to show higher coupling efficiency.^1,2) Therefore, the most suitable activator can be selected according to the substrates and targets.

References

• 1) Acid/Azole Complexes as Highly Effective Promoters in the Synthesis of DNA and RNA Oligomers via the Phosphoramidite Method
  • Y. Hayakawa, R. Kawai, A. Hirata, J. Sugimoto, M. Kataoka, A. Sakakura, M. Hirose, R. Noyori, J. Am. Chem. Soc. 2001, 123, 8165.
• 2) Coupling activators for the oligonucleotide synthesis via phosphoramidite approach
  • X. Wei, Tetrahedron 2013, 69, 3615.

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