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Nile Red Excellent at Detecting Lipid Droplets
Nile Red (1) is a lipophilic fluorescent dye with high photostability. It emits almost no fluorescence in water, but 1 emits strong fluorescence (Exmax: 551 nm, Emmax: 631 nm) upon binding to hydrophobic regions of proteins or lipid droplets.1) 1 can distinctly stain lipid droplets in both live and fixed cells, making it widely used for detecting intracellular lipid droplets using fluorescence microscopy or flow cytometry.2)
Figure. Image of 3T3-L1 cells stained by using 1
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- 1) Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces
- 2) Nile red: a selective fluorescent stain for intracellular lipid droplets
