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Chemiluminescence Enhancer
No.164(January 2015)
Chemiluminescence has been used for analysis in life science, e.g. immunoassay, nucleic acid hybridization assay, Western blotting, etc., as a sensitive and rapid detection method.1) Since Thorpe et al. reported that phenolic compounds such as 4-iodophenol enhance chemiluminescence by the peroxidase–luminol–hydrogen peroxide reaction2), many improved methods have been reported.3) Such methods have been called enhanced chemiluminescence (ECL).3)
4-(2-Methyl-4-thiazolyl)phenol (1) and 4-(4,5-diphenyl-1H-imidazol-2-yl)phenol (2) were reported as having higher enhancing efficiency than the early type of enhancers described above.4,5)
References
- 1)Chemiluminescent and bioluminescent techniques
- 2)Phenols as enhancers of the chemiluminescent horseradish peroxidase–luminol–hydrogen peroxide reaction: Application in luminescence-monitored enzyme immunoassays
- 3)Enhanced chemiluminescence enzyme immunoassay
- 4)Stable and general-purpose chemiluminescent detection system for horseradish peroxidase employing a thiazole compound enhancer and some additives
- 5)Lophine derivatives and analogues as new phenolic enhancers for the luminol–hydrogen peroxide–horseradish peroxidase chemiluminescence system
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