Published TCIMAIL newest issue No.197
Maximum quantity allowed is 999
Bitte wählen Sie die Menge aus.
Easy-to-Handle and Safe Phosphitylation Reagent for Syntheses of DNA and RNA Fragments
No.145(March 2012)
Protected 2’-deoxyribo and ribonucleoside-3’-phosphoramidites, which have been widely used for syntheses of DNA and RNA fragments, are prepared from N,O-diprotected nucleosides and a phosphitylation reagent with an activator such as 1H-tetrazole. 2-Cyanoethyl N,N,N',N'-tetraisopropylphosphordiamidite (1) has been the reagent of frequent choice for phosphitylation due to its ease in handling and safety.1)
References
- 1)Synthesis of protected nucleoside-3'-phosphoramidites using 2-Cyanoethyl N,N,N',N'-tetraisopropylphosphordiamidite
- a)J. Nielsen, M. Taagaard, J. E. Marugg, J. H. van Boom, O. Dahl, Nucl. Acids Res. 1986, 14, 7391.
- b)I. Okamoto, K. Shohda, K. Seio, M. Sekine, J. Org. Chem. 2003, 68, 9971.
- c)A. Misra, S. Mishra, K. Misra, Bioconjugate Chem. 2004, 15, 638.
- d)R. Gukathasan, M. Massoudipour, I. Gupta, A. Chowdhury, S. Pulst, S. Ratnam, Y. S. Sanghvi, S. A. Laneman, J. Organomet. Chem. 2005, 690, 2603.
- a)J. Nielsen, M. Taagaard, J. E. Marugg, J. H. van Boom, O. Dahl, Nucl. Acids Res. 1986, 14, 7391.
- 2)Multibenzoylated nucleosides for synthesis of small oligonucleotides
Related Compounds
- N,O-Protected nucleosides for DNA and RNA fragments synthesis
- B3103
- N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine
- B3087
- N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine
- D3566
- 5'-O-(4,4'-Dimethoxytrityl)thymidine
- I0697
- N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyguanosine
- B3101
- N6-Benzoyl-2'-deoxyadenosine Hydrate
- B3102
- N4-Benzoyl-2'-deoxycytidine
- I0700
- N2-Isobutyryl-2'-deoxyguanosine
- B3093
- N6-Benzoyladenosine
- B3094
- N4-Benzoylcytidine
- I0699
- N2-Isobutyrylguanosine Monohydrate
- B3460
- N6-Dibenzoyladenosine 2',3'-Dibenzoate
- Activators
- B3020
- 5-(Benzylthio)-1H-tetrazole
- D2026
- 4,5-Dicyanoimidazole
- E0670
- 5-(Ethylthio)-1H-tetrazole
- T1017
- 1H-Tetrazole
The prices are subject to change without notice. Please confirm the newest price by our online catalog before placing an order.
In addition, sales products changes with areas. Please understand that a product is not available when the product details page is not displayed.
In addition, sales products changes with areas. Please understand that a product is not available when the product details page is not displayed.