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Imaging is an indispensable experimental technique for observing and analyzing biological phenomena. TCI offers a wide range of reagents from highly versatile fluorescent labeled probes to clearing reagents for animals and plants.
Tissue Clearing Reagents
Tissue clearing is fast becoming one of the main methods to observe the inner structure of deep tissue. TCI offers a wide range of clearing reagents for use with both animals and plants.
Adult mouse whole-brain before and after tissue clearing with CUBIC
Arabidopsis thaliana before and after tissue clearing with iTOMEI
Learn more about Animal Tissue-Clearing Reagents CUBIC
Learn more about Rapid Transparency Reagent for Plants: TOMEI
Learn more about Plants Tissue-Clearing Reagent Suitable for Fluorescent Protein Observation: iTOMEI
Fluorescence Probes for Detection
We have a wide variety of fluorescent dye-labeled secondary antibodies, as well as fluorescent dyes that are pre-weighed and pre-dissolved in solvent for ease of use.
Cells stained with TCI fluorescence probes
Learn more about Fluorescence Probes for Detection
Lipid Droplet Staining Dyes
Lipid droplets are cellular organelles used to store lipids such as triglycerides and cholesterol. There is growing evidence for their roles in autophagy1) and cellular senescence.2)
ShoyakuGreen (= TFMAQ-8Ph) is a green fluorescent dye (λex, max = 404 nm, λem, max = 473 nm) which not only has a high specificity for fat droplets but also low cytotoxicity.
PC6S emits strong green fluorescence (λex, maxem, max = 535 nm) and can dye clearly not only cellular lipid droplets but also tissue lipid droplets.
Oil red O is a diazo dye which has been widely used for staining fat cell and lipids, and stains low polar lipids red.
3T3-L1 cell stained with ShoyakuGreen and PI
HeLa cell stained with PC6S, Hoechst® 33342 and MitoTracker® Red
3T3-L1 cell stained with Oil red O
References
- 1) Autophagy regulates lipid metabolism
- 2) Inhibition of endothelial p53 improves metabolic abnormalities related to dietary obesity
Hoechst® is a registered trademark of Hoechst GmbH. MitoTracker® is a registered trademark of Molecular Probes, Inc.
Cyanine Dyes
Light in the near-infrared region has high tissue permeability, underlying its application in deep tissue imaging in vivo. TCI offers near-infrared fluorochromes with absorption and emission wavelengths in the near-infrared region, such as Indocyanine Green (ICG). We are also happy to discuss bulk synthesis by request.
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Fluorescence excitaion/emission spectrum of Indocyanine Green
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Fluorescence excitaion/emission spectrum of ICG Carboxylic Acid
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Fluorescence excitaion/emission spectrum of IR 754 Carboxylic Acid