Linear PEI HCl [1mg/mL in PBS(-)] [for Transfection]

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Reinheit:

Synonyme

• Linear Polyethyleneimine Hydrochloride [1mg/mL in PBS(-)]

Produktdokumente:

SDS | Spezifikation | AZ & andere Zertifikate | Analytische Diagramme

Generelle Informationen | Spezifikationen und Eigenschaften | Sicherheit und Vorschriften | Anwendungen & Literatur

Ergänzende Produktinformation

Einheit	Stückpreis	Belgien	Japan*	Menge
1ML	30,00 €	Kontaktieren Sie uns	Kontaktieren Sie uns
10ML	210,00 €	Kontaktieren Sie uns	10

*In Belgien verfügbare Lagerbestände werden in 1 bis 3 Tagen geliefert.
*In Japan verfügbare Lagerbestände werden in 1 bis 2 Wochen geliefert. (unter Ausschluss von regulierten Artikeln und Trockeneislieferungen).

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Description

• This product is supplied as a pH-adjusted, 1 mg/mL stock solution of the linear form of the cationic polymer polyethylenimine (PEI) to be used for the transfection of nucleic acids into living cells.

Directions for Use

• • 1. Prepare two aliquots of OPTI-MEM®. Each should be equivalent to 1/4 the regular culture maintenance volume of your cells.
    2. Into one aliquot, mix an appropriate amount of L0466 (*). Into the other aliquot, mix an appropriate amount of DNA (*).
    3. Combine the two aliquots from step 2 and incubate at 37°C for 20 minutes.
    4. Add an equivalent volume of regular culture medium to the solution from step 3.
    5. Aspirate medium from cells and replace with the medium from step 4.
    6. Allow transfection to proceed for between 6 and 24 hours.
    7. Replace medium and incubate until protein expression is detected.
    8. Use the cells for downstream applications.
    9. Create a standard curve to calculate the MDA concentration in the sample.
  *Before running any transfection experiments, optimal transfection conditions (PEI concentration, N:P ratio) for your specific cell line must be determined. We recommend starting with final PEI concentrations ranging from 1 to 6 µg/mL, and N:P ratios of between 10 and 30.
  †Opti-MEM is a registered trademark of Life Technologies Corporation.

Storage

• Store at 4°C. Use within 6 months of receipt.