O-Glycosidase for core1 (Endo-α and Sialidase mix)

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Reinheit:

Synonyme

• O-Glycosidase for core1 (Endo-α & Neuraminidase mix)

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Ergänzende Produktinformation

Einheit	Stückpreis	Belgien	Japan*	Menge
1VIAL	245,00 €	Kontaktieren Sie uns	10

*In Belgien verfügbare Lagerbestände werden in 1 bis 3 Tagen geliefert.
*In Japan verfügbare Lagerbestände werden in 1 bis 2 Wochen geliefert. (unter Ausschluss von regulierten Artikeln und Trockeneislieferungen).

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Product Information

• Quantity : Endo-α 0.1 U per vial (1 U/mL)
• Sialidase 1.0 U per vial (10 U/mL)
• Product Form : Purified Endo-α and Sialidase, Liquid
• Storage buffer : 20 mM Sodium phosphate, 150 mM NaCl buffer, pH 7.4
• Preservative : None
• Stabilizer : None

Description

• Endo-α
• This Endo-α is a glycoside hydrolase that recognizes the structure of the Galβ1-3GalNAc disaccharide α-linked with a hydroxyl group and it releases Galβ1-3GalNAc (galacto-N-biose) by hydrolysis. The Endo-α gene is derived from Bifidobacterium longum, and its recombinant protein is expressed in Escherichia coli.
• Sialidase
• This Sialidase is a glycoside hydrolase that cleaves the glycosidic linkages of neuraminic acids. The Sialidase acts on the α2-3, α2-6, and α2-8 glycosidic linkages located on terminal sialic acid residues composed of oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrates. This enzyme is derived from Arthrobacter ureafaciens.

Unit Definition

• Endo-α
• One unit (U) of the Endo-α is defined as the amount required to produce 1 μmol of Galβ1-3GalNAc from Galβ1-3GalNAc α-pNP (Product No.G0375) per minute at 37°C under the condition of pH 5.0.
• Sialidase
• One unit (U) of the Sialidase is defined as the amount required to produce 1 μmol of N-acetylneuraminic acid (Neu5Ac) from Neu5Ac-α-4MU (product code: M3031) per minute at 37°C under the condition of pH 5.0.

Assay Conditions

• Endo-α
• Reaction conditions: 50 mM Sodium acetate, pH 5.0
  The reaction mixture includes the enzyme and Galβ1-3GalNAc α-pNP in 20 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding 30 μL of 0.25 M sodium carbonate. Quantification of released pNP is measured by a UV spectrometer.
• Sialidase
• Reaction conditions: 50 mM Sodium acetate, pH 5.0
  The reaction mixture includes the enzyme and Neu5Ac-α-4MU in 40 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding a 4-fold volume of 0.1 M sodium carbonate buffer, pH 10.0. Quantification of released 4-MU is measured by HPLC.

Storage

• Store at -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks. <br>(For long-term use, aliquot and freeze after thawing.)

Related Products

• I1205 Sialidase (Neuraminidase)
• I1206 Sialidase (Neuraminidase) Agarose
• 
  
• Other oligosaccharide-releasing enzymes
• [Endo-type N-glycanase (ENGase)]
• A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
• E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
• G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
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• [Endo-type O-glycanase]
• A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
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• [Endo-type glycoceramidase]
• R0240 rEGCase I
• R0241 rEGCase I assisted by Activator II
• R0242 rEGCase II
• R0243 rEGCase II assisted by Activator II
• 
  
• [Endo-type glycosaminoglycanase]
• K0069 Keratanase II (for hydrolysis of Keratan sulfate)