Fluorescent Sialidase Probe Useful for Detecting Influenza Virus

BTP3-Neu5Ac Na (1) is a fluorescent probe that enables the detection of enzyme activity of an enzyme “sialidase”, which is expressed in influenza virus and the infected cells and catalyzes the removal of sialic acid.^1,2) 1 itself doesn’t have fluorescence but releases insoluble BTB3, which is produced by sialidase and emits green fluorescence by UV irradiation. Therefore, selective imaging of drug-resistant influenza viruses is possible when used in combination with 1 and specific sialidase inhibitors, such as zanamivir and oseltamivir.³⁾

References

• 1) Histochemical fluorescent staining of Sendai virus-infected cells with a novel sialidase substrate
• M. Takano, T. Takahashi, T. Agarikuchi, Y. Kurebayashi, A. Minami, T. Otsubo. K. Ikeda, H. Kanazawa, T. Suzuki, Virology 2014, 464-465, 206.
• 2) Imaging of influenza virus sialidase activity in living cells
• Y. Kurebayashi, T. Takahashi, T. Otsubo, K. Ikeda, S. Takahashi, M. Takano, T. Agarikuchi, T. Sato, Y. Matsuda, A. Minami, H. Kanazawa, Y. Uchida, T. Saito, Y. Kawaoka, T. Yamada, F. Kawamori, R. Thomson, M. Itzstein, T. Suzuki, Sci. Rep. 2014, 4, 4877.
• 3) High-Efficiency Capture of Drug Resistant-Influenza Virus by Live Imaging of Sialidase Activity
• Y. Kurebayashi, T. Takahashi, C. Tamoto, K. Sahara, T. Otsubo, T. Yokozawa, N. Shibahara, H. Wada, A. Minami, K. Ikeda, T. Suzuki, PLoS One 2016, 11, e0156400.

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