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CAS RN: | 产品编码: T4031
Clearing Reagent iCBiofilm-H1 [for Biofilm]
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Product Information
- Size : 8 mL
Description
- Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms. Clearing Reagent iCBiofilm-H1 has been developed to allow for the observation of biofilm structure at thicknesses of more than several tens of micrometers. Simple addition of iCBiofilm-H1 solution to a biofilm renders it transparent within several seconds, allowing for easy observation using light microscopy techniques.
*This product was developed by Associate Prof. Sugimoto of the Jikei University School of Medicine.
Storage
- Store at room temperature. Use within 6 months.
Direction for Use
- Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
- After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
- Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
- Stain using appropriate fluorescence reagents.
- Remove staining reagents and add 500 µL – 1 mL of Clearing Reagent iCBiofilm-H1 (Product No.T4031).
- Observe biofilm using optical microscopy.
Related Products
- T4032 Clearing Reagent iCBiofilm-H2 [for Biofilm]
- I1275 Clearing Reagent iCBiofilm-D [for Biofilm]
- G0068 Glutaraldehyde (ca. 50% in Water, ca. 5.6mol/L)
产品编码 | T4031 |
外观与形状(20°C) | 液体 |
储存温度 ![]() |
室温 (15°C以下阴凉干燥处) |
技术规格
Appearance | Colorless to Almost colorlessclear liquid |
Functionality test | to pass test |
Refractive index | 1.3950 to 1.4050 |
物性(参考值)
GHS
相关法规
运输信息
HS编码* | 2924.29-000 |
应用
Clearing of biofilm
Reference
- Instantaneous Clearing of Biofilm (iCBiofilm): an optical approach to revisit bacterial and fungal biofilm imaging
应用
Microscopic observation of biofilm
References
- Light and Electron Microscopic Studies of Microorganisms Growing in Rotating Biological Contactor Biofilms
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