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CAS RN: | Product Number: T4003

Tissue-Clearing Reagent iTOMEI-M (RI 1.40) [for Plants]

× Tissue-Clearing Reagent iTOMEI-M (RI 1.40) [for Plants]

Purity:
Synonyms:
Product Documents:
SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications

Supplemental Product Information
5ML
$32.00
 1   1   17  
25ML
$87.00
 Contact Us 1   8  

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Supplemental Product Information:

"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product can be used as a mounting agent in iTOMEI. Its refractive index is 1.40, optimized to silicone immersion objectives.

Please see a product brochure (PDF) of how to use.


Description

This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene. The refractive index (RI) of this product is optimized to silicone immersion objectives.
The decoloring solution is also required to complete the iTOMEI method.


Storage

The product should be stored at 4 °C.


Directions for Use

Required reagents

  • Paraformaldehyde (PFA)
  • PBS
  • Stains* (option)

==Process outline (All operations are performed at Room temperature) ===============

  • Tissue fixation
    Reagent : 1% PFA in PBS
    Time : 1hr
  • PBS Wash ×3
  • Decoloring
    Reagent : iTOMEI-D (Product No. T3940)
    Time : 24hr
  • PBS Wash ×3
  • (Optional process Staining*, PBS Wash ×3)
  • Clearing (RI matching)**
    Reagent : iTOMEI-M (Product No. T4003)
    Time : Operation time is determined by the mounting solution.
  • Mounting**
    Reagent : iTOMEI-M (Product No. T4003)

*The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.
** Please use the suitable mounting solution for objective lens.
(iTOMEI-M is optimized to silicone immersion objectives.)


Related Products

P0018 Paraformaldehyde
A2412 DAPI·2HCl
E0197 Evans Blue
A0132 Acridine Orange
A0321 Tris(hydroxymethyl)aminomethane
G0316 Glycerol [for Electrophoresis]
S0111 D-(+)-Sucrose
T3530 Tissue-Clearing Reagent TOMEI [for Plants]
T3940 Tissue-Clearing Reagent iTOMEI-D [for Plants]

Product Number T4003
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Light Sensitive,Heat Sensitive
Specifications
Appearance Colorless to Almost colorlessclear liquid
Functionality test to pass test (Remaining fluorescence strength)
Properties (reference)
Specific Gravity (20/20) 1.23
Refractive Index 1.40
GHS
Related Laws:
Transport Information:
HS Number 3822.19.0080
Application
iTOMEI: Plants Tissue-Clearing Method Suitable for Fluorescent Protein Observation

References


Articles/Brochures

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