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CAS RN: | Product Number: O0561
Organosilica FITC (100nm Diam.)
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 2MG |
$732.00
|
Contact Us | 1 | 11 |
|
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Supplemental Product Information:
Product Information
- Volume/Quantity : 2 mg
- Product Form : Suspension in water
- Functional Group : Thiol group (-SH)
- Excitation / Emission : 492 nm / 523 nm
Application
- For use as a fluorophore in the fluorescent labeling of proteins and other molecules. Incorporation of FITC fluorescent dye into conjugatable silica nanoparticles allows for superior fluorescence intensity and light resistance when compared to unmodified FITC. Thiol groups (-SH) at the surface of the nanoparticles provide a convenient mechanism for conjugation. This product is made without toxic metals.
Storage
- Store at 4 °C. Do not freeze.
Direction for Use
- Example of conjugation;
Organosilica conjugation with Mouse IgG antibody (M.W. 150,000)- Prepare 10 mM linker solution (For examples of linkers, see Related Products).
e.g. Dissolve 2.8 mg of GMBS (Product No. S0399) in 1 mL of DMSO (Product No. D0798). - Mix 10 mM linker solution with appropriate volume of antibody. (Suggested linker:antibody molar ratio = 100:1)
e.g. Mix 100 µL of 1.5 mg/mL (= 10 µM) antibody solution with 10 µL of 10 mM linker solution. - Incubate linker-antibody solution for 1 hour at room temperature.
- Remove unreacted and/or hydrolyzed reagent using a desalting column or dialysis method (not included).
- Mix 1 part by weight purified antibody-linker solution with 1.5 parts by weight Organosilica FITC (Product No.O0561).
e.g. For the antibody-linker solution described in step 2 (antibody weight approx. 0.15 mg), use 45 µL of 5.0 mg/mL Organosilica FITC. - Incubate the antibody-nanoparticle solution for 1 hour at room temperature.
- Collect conjugated nanoparticles via centrifugation (20,000 x g, 4 °C, 10 minutes) and wash with 500 µL deionized water. Repeat wash step two additional times. Resuspend final washed pellet in deionized water.
- Prepare 10 mM linker solution (For examples of linkers, see Related Products).
Related Products
- [Linkers for Thiol-Amine Conjugation]
- S0399 N-Succinimidyl 4-Maleimidobutyrate
- S0427 N-Succinimidyl 3-Maleimidopropionate
- S0861 N-Succinimidyl 4-(4-Maleimidophenyl)butyrate
- S0882 N-Succinimidyl 11-Maleimidoundecanoate
- [Related Special Fluorescent Products]
- O0573 Organosilica Rhodamine B 100nm
- B5419 BMeS-p-A (extremely small fluorescent dye)
| Product Number | O0561 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
Specifications
| Appearance | Light yellow to Amber to Dark green clear liquid to cloudy liquid |
| Functionality test | to pass test (thiol detection test) |
Properties (reference)
GHS
Related Laws:
Transport Information:
| HS Number | 3824.99.9397 |
Application
Conjugation to Protein A
We labeled organosilica FITC [O0561] with Protein A and measured the fluorescence intensity when it reacted with Mouse IgG.
Stronger fluorescence than that of the conventional FITC-labeled product was observed.
References
- Imaging of size-dependent uptake and identification of novel pathways in mouse Peyer's patches using fluorescent organosilica particles
- Size-controlled synthesis, surface functionalization, and biological applications of thiol-organosilica particles
- Near-Infrared Fluorescent Thiol-Organosilica Nanoparticles That Are Functionalized with IR-820 and Their Applications for Long-Term Imaging of in Situ Labeled Cells and Depth-Dependent Tumor in Vivo Imaging
Application
Organosilica FITC 100nm
References
- Histochemical and biochemical analysis of the size-dependent nanoimmunoresponse in mouse Peyer’s patches using fluorescent organosilica particles
- Size-Controlled, One-Pot Synthesis, Characterization, and Biological Applications of Epoxy-Organosilica Particles Possessing Positive Zeta Potential
Articles/Brochures
TCIMAIL
[Featured Products] Fluorescent Organosilica Nanoparticles[Product Highlights] Fluorescent Organosilica Nanoparticles for Biological Imaging
Product Documents (Note: Some products will not have analytical charts available.)
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Specifications
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Other Documents
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