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CAS RN: 31282-04-9 | Product Number: H1509
Hygromycin B
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Purity: >80.0%(HPLC)
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
|---|---|---|---|---|
| 200MG |
€43.00
|
7 | 21 |
|
| 1G |
€135.00
|
15 | ≥60 |
|
*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).
| Product Number | H1509 |
| Purity / Analysis Method | >80.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__2__0H__3__7N__3O__1__3 = 527.52 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
| Packaging and Container | 1G-Glass Bottle with Plastic Insert (View image), 200MG-Glass Bottle with Plastic Insert (View image) |
| CAS RN | 31282-04-9 |
| Reaxys Registry Number | 66457 |
| PubChem Substance ID | 253661073 |
| Merck Index (14) | 4852 |
| MDL Number | MFCD06795479 |
Specifications
| Appearance | White to Light yellow to Light orange powder to crystal |
| Purity(HPLC)(CAD) | min. 80.0 area% |
| Specific rotation [a]20/D | +18.0 to +23.0 deg(C=1, H2O) |
Properties (reference)
| Melting Point | 180 °C(dec.) |
| Specific Rotation | 20° (C=1,H2O) |
GHS
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H300 + H310 + H330 : Fatal if swallowed, in contact with skin or if inhaled. H318 : Causes serious eye damage. H317 : May cause an allergic skin reaction. H334 : May cause allergy or asthma symptoms or breathing difficulties if inhaled. |
| Precautionary Statements | P260 : Do not breathe dust. P262 : Do not get in eyes, on skin, or on clothing. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P342 + P311 : If experiencing respiratory symptoms: Call a POISON CENTER/doctor. P301 + P310 + P330 : IF SWALLOWED: Immediately call a POISON CENTER/doctor. Rinse mouth. P302 + P352 + P310 : IF ON SKIN: Wash with plenty of water. Immediately call a POISON CENTER/doctor. P304 + P340 + P310 : IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor. P305 + P351 + P338 + P310 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor. P403 + P233 : Store in a well-ventilated place. Keep container tightly closed. |
Related Laws:
| EC Number | 250-545-5 |
| RTECS# | WK2130000 |
Transport Information:
| UN Number | UN3462 |
| Class | 6.1 |
| Packing Group | I |
| HS Number | 2940000080 |
Application
Hygromycin B: A Selective Reagent in Molecular Genetics Experiments on Cells
Hygromycin B is a water-soluble aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus. It is acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. In molecular genetics, hygromycin B is used in genetic modification as a selective reagent to isolate prokaryotic and eukaryotic cells coupled to a gene coding for hygromycin resistance.
References
- Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae
- Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells
- Resistance to hygromycin B. A new marker for plant transformation studies
- Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli
- Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene
- Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method
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