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CAS RN: | Product Number: R0238

rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]


Purity:
Synonyms:
  • rSRL-Agarose
  • Recombinant Sclerotium rolfsii lectin (= rSRL)-Agarose
Product Documents:
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Supplemental Product Information:

Product information
Product code : R0238
Product name : rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
Preparation : Recombinant SRL is covalently coupled with agarose gel
Quantity : 1 ml gel/2 ml (1:1, v/v slurry) suspended in PBS, pH7.5
Protein concentration : about 15 mg/ml gel
Binding capacity : >7.5 mg of glycoprotein (agalacto human Transferrin) /ml gel
Preservative : 0.02% [w/v] Sodium azide
Stabilizer : None

Description
The rSRL is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. This lectin preferably binds to [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] glycan epitopes by its two distinct carbohydrate-binding sites.
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rSRL-LecBeads (1 ml gel) shows a binding capacity of about 7.5 mg or more agalacto human Transferrin [agalacto hTF] through its binding to a terminal [GlcNAcβ(1-2)Man] glycan epitope on agalacto hTF.

Storage
Store at 2-10°C. (Avoid freezing).

Related Products
R0228 Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0236 rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc
R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0239 rGRFT-LecBeads [for Manα(1-2)Man]
A3331 AOL-LecBeads [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]

Product Number R0238
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Heat Sensitive
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