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Ready-to-use Solution for Protein Extraction from Nervous Tissue

 1 is a ready-to-use solution for protein extraction from nervous tissue. By suspending tissue in 1 and then centrifuging, the supernatant containing proteins can be obtained. Extracted protein can be used in downstream applications such as electrophoresis and western blotting.

Application example

[Protein extraction]

  1. Wash mouse brain twice with PBS.
  2. Weigh samples. For each gram of sample, add 10 mL of 1 and homogenize.
  3. Incubate on ice for 10 minutes.
  4. Centrifuge the sample at 10000 × g for 10 minutes at 4 °C and collect lysate fraction.

[Protein quantification]: measurement of protein concentration in lysate fraction by pyrogallol red method

Title

[Electrophoresis / Western blotting]: Detection of specific protein in lysate fraction

Title

Synaptophysin (38 kDa) in synaptic vesicle can be extracted from mouse brain efficiently.

References

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