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Peroxidase Detection

S0887
Chemiluminescence has contributed to providing high sensitivity for enzyme-linked immunoassays.1,2) Thorpe et al. referred that, when peroxidase is used for immunoassay as a labeled enzyme, hydrogen peroxide can be replaced by oxidants such as perborate.2) Compound 1 is the sodium salt of perborate. In their study,3) they used the sodium salt to replace hydrogen peroxide. 1 has already been employed for recent improvements of immunoassay.4,5)
On the other hand, interestingly, 1 has already been used as a substrate of catalase in the late 1940’s.6)

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