Maintenance Notice (10:30 PM January 4 - 8:00 AM January 5, 2025 UTC): This website is scheduled to be unavailable due to maintenance. We appreciate your patience and understanding.
Published TCIMAIL newest issue No.197 | Notice of Discontinuing the Use of Password-Protected Compressed Files | Various analytical charts can be searched on each product detail page and Product Document Search (The kinds of analytical charts differ by product)
Maximum quantity allowed is 999
请选择数量
Buffer and Additives for LC Analysis of Oligonucleotides
Triethylammonium acetate solution is broadly utilized as an RP-HPLC buffer for the purification/analysis of oligonucleotides.1) It is also known that triethylamine/1,1,1,3,3,3-hexafluoroisopropyl alcohol (= HFIP) buffer improves ESI sensitivity in the detection of oligonucleotide LC-MS analysis.2)
TCI guarantees, that for triethylammonium acetate solution (1), triethylamine (2) and 1,1,1,3,3,3-hexafluoroisopropyl alcohol (3) no UV absorption occurs in the analytical range of oligonucleotide. The suitable buffer solutions can be prepared by diluting 1 or mixing/diluting required amount of 2 and 3, respectively.
Related Products
- T4022
- Triethylammonium Acetate (2.0mol/L in Water)
- T4021
- Triethylamine [for HPLC]
- H1793
- 1,1,1,3,3,3-Hexafluoroisopropyl Alcohol [for HPLC]
References
- 1) Separation of small DNA and RNA oligonucleotides by high-performance anion-exchange liquid chromatography
- 2) Analysis of oligonucleotides by HPLC-electrospray ionization mass spectrometry
