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CAS RN: | 產品號碼: T3854
TMB [for ELISA] (Ready-to-use solution)
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補充產品訊息:
Product Information
Volume : 100 mL
Description
This product is supplied as a ready-to-use solution containing 3,3',5,5'-Tetramethyl -benzidine (TMB) and hydrogen peroxide for ELISA. When this product reacts with horseradish peroxidase (HRP), a blue colored soluble reaction product appears. Its absorbance can be measured at 650 nm. After terminating the reaction by adding hydrochloric acid, the reaction product becomes yellow and its absorbance can be measured at 450 nm.
Direction for Use
The following steps are for a 96-well plate format.
1. Bring TMB solution (Product No. T3854) to room temperature.
2. Gently mix the TMB solution (Product No. T3854).
3. Add 100uL of TMB solution (Product No. T3854) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. To terminate the reaction, add 100uL of 1 N HCl (Product No. H1202) to each well.
6. Within 1 hour of terminating the reaction, measure the absorbance of each well at 450 nm.
Storage
The product should be stored at 4 ºC, and used within 6 months.
Precipitations sometimes arise, but they do not affect the quality.
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| 產品號碼 | T3854 |
| 外觀與形狀（20°C） | Liquid |
| 儲存條件 | Refrigerated (0-10°C) |
| 應避免的情況 | Heat Sensitive |
產品規格
| Appearance | Colorless to Almost colorlessclear liquid |
| Peroxidase detection test | to pass test |
性質
GHS
| 圖形表示 |
|
| 信號詞 | Danger |
| 危險性說明 | H370 : Causes damage to organs. H372 : Causes damage to organs through prolonged or repeated exposure. H227 : Combustible liquid. |
| 防範說明 | P501 : Dispose of contents/ container to an approved waste disposal plant. P270 : Do not eat, drink or smoke when using this product. P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor. P403 : Store in a well-ventilated place. P405 : Store locked up. |
相關法規
運輸資料
| HS編碼* | 3822.19-000 |
Application
TMB [for ELISA] (Ready-to-use solution)
References
- Cold Spring Harbor Laboratory Press, New York
- E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p404, p506, p594
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