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CAS RN: | 產品號碼: R0229
Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
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補充產品訊息:
[Handling Notes]
Avoid multiple freeze-thaw cycles. Add some preservative, e.g. sodium azide upon opening for the first time, and store at 2-10 °C.
Product Information
Product code: R0229
Product name: Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Purified recombinant GRFT, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: 13 kDa
Preservative : None
Stabilizer: None
Description
The rGRFT is a carbohydrate-binding protein (Lectin) derived from Griffithsia sp. The rGRFT gene product is expressed in Escherichia coli. This lectin preferably binds to a [Manα(1-2)Man] glycan epitope. Consequently, rGRFT exhibits a hemagglutination activity to a guinea pig (but not rabbit) reticulocyte through the glycan binding to subsites and subunits of the lectin.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it's recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
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| 產品號碼 | R0229 |
| 外觀與形狀（20°C） | Liquid |
| 儲存條件 | Frozen (-20°C) |
| 應避免的情況 | Heat Sensitive |
產品規格
| Appearance | Colorless to Light yellow to Light orange clear liquid |
| Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
| Molecular weight | 10000 to 25000(SDS-PAGE) |
| Hemagglutination activity | max. 16 micro g/mL(pH7.2) |
性質
GHS
相關法規
運輸資料
| HS編碼* | 3504.00-000 |
Application
Recombinant lectin
References
- Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp
- Recombinant production of anti-HIV protein, griffithsin, by auto-induction in a fermentor culture
- Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding
TCIMail
[TCIMAIL No.188] Recombinant Antiviral Lectin GRFT[TCIMAIL No.193] Hemagglutinin: Recombinant Lectins
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