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CAS RN: | 產品號碼: R0228
Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
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補充產品訊息:
[Handling Notes]
Avoid multiple freeze-thaw cycles. Add some preservative, e.g. sodium azide upon opening for the first time, and store at 2-10 °C.
Product Information
Product code: R0228
Product name: Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Purified recombinant SRL, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: 16 kDa
Preservative : None
Stabilizer: None
Description
The rSRL is a carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. The rSRL gene product is expressed in Escherichia coli. This lectin preferably binds to two types [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] of glycan epitopes by its two distinct carbohydrate-binding sites. Consequently, rSRL exhibits a hemagglutination activity to a rabbit reticulocyte through the glycan binding to subsites and subunits of the lectin.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it's recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
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| 產品號碼 | R0228 |
| 外觀與形狀（20°C） | Liquid |
| 儲存條件 | Frozen (-20°C) |
| 應避免的情況 | Heat Sensitive |
產品規格
| Appearance | Colorless to Light yellow to Light orange clear liquid |
| Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
| Molecular weight | 10000 to 25000(SDS-PAGE) |
| Hemagglutination activity | max. 12.5 micro g/mL(pH7.2) |
性質
GHS
相關法規
運輸資料
| HS編碼* | 3504.00-000 |
Application
Recombinant lectin
References
- A newly isolated lectin from the plant pathogenic fungus Sclerotium rolfsii: purification, characterization and role in mycoparasitism
- Carbohydrate specificity of a lectin isolated from the fungus Sclerotium rolfsii
- Structural basis for the carbohydrate recognition of the Sclerotium rolfsii lectin
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