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CAS RN: | 產品號碼: N1209
10X Nucleic Acid Stain Blue [for Electrophoresis]
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補充產品訊息:
Product Information
Volume : 100 mLStorage
Store at room temperature.
Description
This product can be used to stain nucleic acids following agarose gel electrophoresis.
As this product stains nucleic acids blue, no detection device is required. Unlike common nucleic acid staining reagents such as ethidium bromide it is non-mutagenic, making it very safe and easy to handle.
Directions for Use
Nucleic Acid Stain Blue is provided as a 10x concentrate. To use, dilute by mixing one part of Nucleic Acid Stain Blue with nine parts of lab-grade water (e.g. add 10 mL of 10x concentrate to 90 mL water).
In most cases, a 10 minute staining time* is adequate to visualize samples.
Once staining is complete, incubate in lab-grade water to remove unbound dye.**
Diluted Nucleic Acid Stain Blue can be used several times within a few days to a week, depending on how often it is used.
*Enough staining solution should be used to completely cover the gel.
**It is possible to decolorize more quickly by using warm water (below 60°C) and/or by frequently replacing the dye-extracted old water.
Related Products
E1363 Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]L0393 6X Loading Buffer Bromophenol Blue [for Electrophoresis] [for Molecular Biology] (1 mLx3)
| 產品號碼 | N1209 |
| 外觀與形狀（20°C） | Liquid |
| 儲存條件 | Room Temperature (Recommended in a cool and dark place, <15°C) |
產品規格
| Appearance | Blue to Dark blue clear liquid |
| DNA staining test | to pass test |
性質
GHS
相關法規
運輸資料
| HS編碼* | 3204.13-000 |
Application
DNA Staining Reagents for Agarose Gel Electrohporesis
Figure 1. Agarose gel after staining with ethidium bromide (0.5 mg/mL solution) (taken in-house) 
Figure 2. Agarose gel after staining with 10X Nucleic Acid Stain Blue (taken in-house)
References
- Agarose Gel Electrophoresis for the Separation of DNA Fragments
- Detection of DNA in Agarose Gels
- J. F. Sambrook, D. W. Russell, in Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, New York, 2001, Vol. 1, Chap. 5, 14.
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