MTT Solution [for Cell proliferation assay] (1mL×5)

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纯度/分析方法:

別名

• 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium Bromide Solution (1mL×5)

文件：

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補充產品訊息

包裝	價格	同一天	2-3个工作日	左側以上貨期	貨期資訊
1SET	NT$3,200	≥60	0	聯繫我們	貨期預估

* 以上價格已含運費關稅等但一些需要海運以及乾冰運輸的產品除外,詳情請與 當地經銷商 洽詢。
* TCI會時常優化儲存條件,儲存溫度請以在線目錄為準，敬請留意。

大包裝詢價

Product Information

• Volume/Quantity : 1mL x 5
• Product Form : 5.0 mg/mL in PBS

Description

• 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide (MTT) is a tetrazolium salt that is converted to formazan when taken up by living cells. Although formazan forms crystals in cells due to its insolubility in water, it is soluble in DMSO. By dissolving these formazan crystals in DMSO and reading the resultant absorbance at 540 nm, an estimate of cell number can be obtained. This product comprises a solution of 5.0 mg/mL MTT dissolved in PBS. To use, simply apply 1/10 of the culture medium volume of MTT Solution to live cells.

Storage

• Stored at -20 °C and protected from light. Avoid repeated freezing and thawing.

Direction for Use

• MTT solution can be added at any point in the cell culture. For best results, it should be added to cells in the logarithmic growth phase. Pre-determine the number of cells and seed 5,000 to 50,000 cells per well in a 96-well plate.

• 1. Seed 100 µL of cells in a 96-well plate.
  2. Incubate overnight in an incubator.
  3. Add 10 µL of MTT solution to each well.
  4. Incubate for 2-4 hours for color reaction.
  5. Remove the medium, taking care not to suck up the formazan.
  6. Add 100 µL of DMSO (Product No. D0798) and dissolve the formazan.
  7. Measure the absorbance at 540 nm.

Related Products

• R0195 Resazurin (Ready-to-use solution) [for Cell proliferation assay]
• D0798 Dimethyl Sulfoxide
• M3297 MTT [for Biochemical Research]