Maximum quantity allowed is 999
请选择数量
CAS RN: | 產品號碼: D5909
補充產品訊息:
Product Information
Components : DAB solution, Hydrogen Peroxide solution, 1.5M Tris-HCl buffer
Size : 100 tests (About 500 mL substrate solution)
Description
This kit consists three reagents; 3, 3’-diaminobenzidine (DAB) solution, Hydrogen Peroxide solution and 1.5M Tris-HCl buffer and it is supplied to detect horseradish peroxidase (HRP) activity in Western blotting and so on. When DAB reacts with HRP, a brown precipitate appears. This kit does not include nickel component.
Direction for Use
1) Prepare a blotting membrane covered with HRP-conjugated antibody.
2) Prepare the DAB substrate solution according to the following volume;
- Add 5 drops of DAB solution (about 140 µL)
- Add 2 drops of Hydrogen Peroxide solution (about 80 µL)
- Add 2 drops of 1.5M Tris-HCl buffer (about 80 µL)
- Each reagent is added into 5 mL of deionized water
3) Soak the membrane in the DAB substrate solution and incubate until the membrane is colored.
4) Add deionized water and stop the coloring reaction.
Storage
The product should be stored at 4 °C and used within 6 months.
| 產品號碼 | D5909 |
| 外觀與形狀（20°C） | Liquid |
| 儲存條件 | Refrigerated (0-10°C) |
| 應避免的情況 | Heat Sensitive |
產品規格
性質
GHS
相關法規
運輸資料
| HS編碼* | 3822.19-000 |
Application
DAB Staining Kit to Detect Peroxidase
Figure. Dot blot image of substrate solutions prepared by DAB staining kit reacting with HRP (from left to right: 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078 (mU/spot)).
References
- Assessment of a method for immunochemical detection of antigen on nitrocellulose membranes.
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
Application
Staining using DAB as a chromogenic substrate for peroxidase
References
- Studies on Microperoxisomes V. Are Microperoxisomes Ubiquitous in Mammalian Cells?
文章/手冊
產品文件 （部分產品的分析圖譜無法提供，敬請諒解。）
SDS
請選擇語言。
請求的SDS不可用。
如需更多幫助，請聯繫我們 。
產品規格
檢驗報告(CoA)及其他文檔
請輸入批號
輸入的批號不正確
示例 CoA
可下載CoA示例。注：該示例可能非最新批次的CoA。
目前沒有該產品的 CoA 示例。
分析圖譜
請輸入批號
輸入的批號不正確
很抱歉，您搜索的分析圖譜無法提供。