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CAS RN: | 產品號碼: B6105

6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]


纯度/分析方法:
別名
文件:
10ML
NT$2,000
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* 以上價格已含運費關稅等但一些需要海運以及乾冰運輸的產品除外,詳情請與 當地經銷商 洽詢。
* TCI會時常優化儲存條件,儲存溫度請以在線目錄為準,敬請留意。


補充產品訊息:

Product Information

Volume : 10 mL

Description

This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
Note: Dissolve the precipitation at 25 - 30 °C if precipitation occurs during storage.


Description for Use

Preparation of non-reducing sample
A protein sample is mixed with the 6X sample buffer (5:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.

Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 30 μL of 2-Mercaptoethanol per 70 μL of 6X sample buffer. Mix thoroughly.
Then a protein sample is mixed with the sample buffer (5:1) and heating to 95 - 100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (TCI Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (TCI Product No. D3647), etc.


Storage

Store at room temperature.


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B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]
B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
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產品號碼 B6105
外觀與形狀(20°C) Liquid
儲存條件 Room Temperature (Recommended in a cool and dark place, <15°C)
產品規格
Appearance Green to Dark green to Dark blue clear liquid to cloudy liquid
Functionality test to pass test(Electrophoresis)
性質
比重 1.16
GHS
圖形表示 Pictogram
信號詞 Warning
危險性說明 H315 : Causes skin irritation.
防範說明 P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
相關法規
運輸資料
HS編碼* 3822.19-000
*此H.S.編碼用於日本出口報關, 不適用於您所在國家或地區的進口申報
Application
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

References


產品文件 (部分產品的分析圖譜無法提供,敬請諒解。)
SDS
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產品規格
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