Maximum quantity allowed is 999
CAS RN: | 產品號碼: B6105
6X Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
* 以上價格已含運費關稅等但一些需要海運以及乾冰運輸的產品除外,詳情請與
當地經銷商
洽詢。
* TCI會時常優化儲存條件,儲存溫度請以在線目錄為準,敬請留意。
補充產品訊息:
Product Information
Volume : 10 mLDescription
This product contains 12% LDS, 50% glycerol, 0.03% bromophenol blue and 0.375 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. 6X Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system. LDS is anionic detergent that may be used in place of SDS for electrophoresis, it is more soluble than SDS.
Note: Dissolve the precipitation at 25 - 30 °C if precipitation occurs during storage.
Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 6X sample buffer (5:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 30 μL of 2-Mercaptoethanol per 70 μL of 6X sample buffer. Mix thoroughly.
Then a protein sample is mixed with the sample buffer (5:1) and heating to 95 - 100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (TCI Product No. M1948), DL-Dithiothreitol [for Electrophoresis] (TCI Product No. D3647), etc.
Storage
Store at room temperature.
Related Products
A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]A3218 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
B3193 Coomassie Brilliant Blue G-250 [for Electrophoresis]
B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]
B5834 2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
B6110 2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]
C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
產品號碼 | B6105 |
外觀與形狀(20°C) | Liquid |
儲存條件 | Room Temperature (Recommended in a cool and dark place, <15°C) |
Appearance | Green to Dark green to Dark blue clear liquid to cloudy liquid |
Functionality test | to pass test(Electrophoresis) |
比重 | 1.16 |
圖形表示 |
![]() |
信號詞 | Warning |
危險性說明 | H315 : Causes skin irritation. |
防範說明 | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves. P302 + P352 : IF ON SKIN: Wash with plenty of water. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
HS編碼* | 3822.19-000 |
References
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
- Dodecyl sulfate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.
SDS
請求的SDS不可用。
如需更多幫助,請聯繫我們 。
產品規格
檢驗報告(CoA)及其他文檔
示例 CoA
目前沒有該產品的 CoA 示例。
分析圖譜
很抱歉,您搜索的分析圖譜無法提供。