Clearing Reagent iCBiofilm-H2 [for Biofilm]

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Size	Unit Price	Same Day	2-3 Business Days	Other Lead Time	Shipping Information
8ML	NT$3,640	12	0	Contact Us	Shipping Simulation

* The above prices include freight cost, customs, and other charges to the destination except for products that need to be shipped by sea or dry ice. For details, please contact our distributor in Taiwan to order our product.
* The storage conditions are subject to change without notice.

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Product Information

• Size : 8 mL

Description

• Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms.
  In order to observe the structures of biofilms with a thickness of more than several tens of micrometers, Clearing Reagent iCBiofilm-H2 has been developed. iCBioflim-H2 is supplied as a stock solution. Before adding to biofilm, iCBiofilm-H2 has to be diluted with deionized water to appropriate concentration (undiluted iCBiofilm-H2 may work well). By adding iCBiofilm-H2 solution to a biofilm, the biofilm becomes transparent within several seconds and easy-to-observe by light microscopy such as confocal laser scanning microscopy.
  *This product was developed by Associate Prof. Sugimoto, the Jikei University School of Medicine.

Storage

• Stored at room temperature and used within 6 months.

Direction for Use

• 1. Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
  2. After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
  3. Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
  4. Stain using appropriate fluorescence reagents.
  5. Dilute iCBiofilm-H2 (Product No. T4032) with deionized water to an appropriate concentration.
     Recommendation ratio; iCBiofilm-H2 : water = 2 : 1 or 1 : 1
     If you need the best performance of iCBioflim-H2 for a target biofilm, please consider to examine different concentrations of iCBiofilm-H2.
  6. Remove staining reagents and add diluted or undiluted iCBiofilm-H2 solution (about 500 µL – 1 mL).
  7. Observe the biofilm by optical microscopy.

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