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CAS RN: | Product Number: R0236

rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc]

× rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc]

Purity:
Synonyms:
  • rLSL-N-Agarose
  • Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N)-Agarose
Product Documents:
SDS | Specifications | C of A & Other Certificates | Analytical Charts

General Information | Specifications & Properties | Safety & Regulations | Applications

Supplemental Product Information
1VIAL
NT$16,360
 3   1  

* The above prices include freight cost, customs, and other charges to the destination except for products that need to be shipped by sea or dry ice. For details, please contact our distributor in Taiwan to order our product.
* The storage conditions are subject to change without notice.

Supplemental Product Information:

Product information
Product code : R0236
Product name : rLSL-N-LecBeads [for Galβ(1-4)GlcNAc, poly LacNAc]
Preparation : Recombinant LSL-N is covalently coupled with agarose gel
Quantity : 1 ml gel/2 ml (1:1, v/v slurry) suspended in PBS, pH7.5
Protein concentration : about 10 mg/ml gel
Binding capacity : >12 mg of glycoprotein (asialo human Transferrin) /ml gel
Preservative : 0.02% [w/v] Sodium azide
Stabilizer : None

Description
The rLSL-N is an Escherichia coli recombinant of N-terminal domain of carbohydrate-binding protein (Lectin) derived from Laetiporus sulphureus. This lectin preferably binds to [Galβ(1-4)GlcNAc, poly LacNAc] glycan epitopes (It very slightly interacts with lactose).
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This LSL-N-LecBeads (1 ml gel) shows a binding capacity of about 12 mg or more asialo human Transferrin [asialo hTF] through its binding to a terminal [Galβ(1-4)GlcNAc] glycan epitope on asialo hTF.

Storage
Store at 2-10°C. (Avoid freezing).

Related Products
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in Escherichia coli
R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
R0235 rPSL1a-LecBeads [for Siaα(2-6)Gal]
R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0239 rGRFT-LecBeads [for Manα(1-2)Man]
A3331 AOL-LecBeads [for Fucα(1-6)/α(1-4)/α(1-3)/α(1-2)]

Product Number R0236
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Heat Sensitive
Specifications
Appearance White to Almost white clear liquid to cloudy liquid
Concentration(Lowry method) min. 9.0 mg/mL gel
Binding capacity to pass test(min. 10 mg/mL gel, Asialo-human transferrin)
Properties (reference)
GHS
Related Laws:
Transport Information:
H.S.code* 3504.00-000
*This code is applied to the products when TCI exports from Japan and not for import in your country.
Application
The lectin-catch procedure

< Preparation >
Buffers
Binding buffer : PBS pH7.5
Elution buffer : PBS pH7.5 containing 200 mM lactose [Product code: L0008] and 0.1% triton-X100 [Product code: P1775]

< Method of the lectin-catch > ※Centrifuge method

Equilibration

  • Prepare the LecBeads in a new tube.
  • Add the Binding buffer into the tube to equilibrate the gel.
  • Centrifuge the tube at 800 ×g for 3 min and discard the supernatant. (If necessary, repeat equilibration with the Binding buffer >10-fold volume of the LecBeads appropriately.)

Binding

  • Concentrate or dilute the glycoprotein sample to moderate concentration (e.g., 1 mg/ml) with the Binding buffer [Input fraction], and apply the prepared sample to the tube in which the LecBeads was preliminary equilibrated.
  • Agitate the tube at 4°C (※Generally, a dissociation constants (Kd value) of lectins are tend to be decreased at lower temperature) for a few hours (or overnight).
  • Centrifuge the tube at 800 ×g for 3 min and remove the supernatant [Through fraction].
  • Wash the LecBeads by 5 (to 10)-fold of the slurry volume with the Binding buffer several times.


Elution

  • Elute the glycoprotein from the LecBeads by agitation with an appropriate volume of the Elution buffer (e.g. same volume of the LecBeads).
  • Agitate the reaction tube at 4°C or R.T. for a few hours (or overnight).
  • Centrifuge the tube at 800 ×g for 3 min and remove the supernatant [Elution fraction].
  • Check the resulting fractions ([Input fraction] [Through fraction] [Elution fraction]) by protein assay and SDS-PAGE. (In some cases, the repetitive elution step with a small volume of the Elution buffer might improve the elution effect. If necessary, repeat elution steps.)
  • The competitive oligosaccharide included in the obtained elution fractions can be removed by ultrafiltration or dialysis using a sufficient amount of buffer.

 

References


Articles/Brochures

TCIMAIL
[TCIMAIL No.192] Glycoprotein Capturing Reagents: Lectin-Immobilized Agarose "LecBeads"
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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Other Documents

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