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CAS RN: | Product Number: H1621
Horseradish Peroxidase Maleimide Conjugate (0.5mg×3)
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Purity:
Synonyms:
- HRP Maleimide (0.5mg×3)
- Horseradish Peroxidase Maleimide Activated (0.5mg×3)
Product Documents:
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Product Information
Volume/Quantity : 0.5 mg x 3 vialConjugation : Peroxidase from Horseradish (HRP) was conjugated to Sulfo-SMCC (Product No. S0883).
Product Form : Horseradish Peroxidase Maleimide Conjugate. Lyophilized in 10 mM sodium phosphate, 0.115 mM NaCl, 40 mM sucrose, pH 6.6.
Preservative : None
Description
HRP Maleimide Conjugate is designed to facilitate the conjugation of the HRP to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups. Activated HRP contains 1–3 maleimide groups for each HRP molecule.
Protocol
- Firstly return the vial to room temperature.
- Reconstitute the contents of the vial with 100μL of buffer or water to obtain a 5 mg/mL solution of HRP Maleimide.
Note: Do not vortex or heat the HRP Maleimide. - Dissolve the sulfhydryl compound to be conjugated at 2-5 mg/ml in the buffer.
Note: We recommend that the thiol-containing molecule is dissolved in the pH range 6.5-7.5 buffer. 0.1M sodium phosphate, 0.15M NaCl, 10mM EDTA at pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA may help to prevent metal catalysed oxidation of thiols. Use of a 4:1 (HRP Maleimide:antibody) molar ratio in the conjugation reaction usually results in high-activity conjugates. This vial contains 0.5 mg of HRP Maleimide Conjugate and can effectively modify 0.5 mg of immunoglobulin. - Immediately mix the thiol-containing molecule and HRP Maleimide solutions and allow them to react for 2 hours at room temperature. To increase HRP incorporation, extend reaction time up to 12 hours.
- If desired, desalt, dialyze or purify the conjugate.
Storage
Store at −20 °C to −80 °C. Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
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| Product Number | H1621 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature | Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Light yellow to Brown powder to crystal |
| Molar Ratio Maleimido | 1-3 moles of maleimide per mole of HRP |
Properties (reference)
GHS
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Transport Information:
| H.S.code* | 3507.90-000 |
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