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CAS RN: | Product Number: C3709
CUBIC-HV™1 3D nuclear staining kit
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Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time |
Shipping Information
|
|---|---|---|---|---|---|
| 1KIT |
NT$17,480
|
0 | 0 | Contact Us |
* The above prices include freight cost, customs, and other charges to the destination except for products that need to be shipped by sea or dry ice. For details, please contact
our distributor
in Taiwan to order our product.
* The storage conditions are subject to change without notice.
Supplemental Product Information:
This kit contains buffers and typical nuclear stains for 3D nuclear staining of CUBIC-cleared tissues.
Please see the detail protocols in CUBICStars’ web site.
https://www.cubicstars.com/cubic-hv/index.html
Constituents in kit (suitable for a brain of an adult mouse)
- CUBIC-HV™1 3D nuclear staining buffer (1x) (for 10 tests)
- CUBIC-HV™1 3D nuclear staining washing buffer (100x) (for 10 tests)
- DAPI·2HCl (200x) (for 10 tests)
- Propidium Iodide (100x) (for 10 tests)
- 10 x 5 mL tube
Process outline
====Delipidation====
Perfusion fixation and dissection of the mouse brain
Post-fix <24 h
Wash (PBS) 2 h x3
Delipidation (CUBIC-L; Product No. T3740) 3-7 days
Wash (PBS) 2 h x3
====Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3709) ====
Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3-5 days
Wash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3
==== Optional process; Enzyme reaction ====
====Immunostaining by CUBIC-HV™1 3D immunostaining kit (Product No. C3708) ====
Primary antibody (anti-NeuN antibody in C3708 kit can be used): 5 µg/whole brain
Secondary antibody: anti-Fc Fab (out of the kits), 3.75 µg/whole brain
Primary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer, 1.5 h
Immunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)), >1 week
Reaction in the same buffer at 4°C, 1 day
Wash (CUBIC-HV™1 3D immunostaining wash buffer), 2 h x3
====Fix and RI matching====
Post-fix (in 1% Formaldehyde Solution), 1 day
Wash (PBS) 2 h
RI matching (CUBIC-R+; Product No. T3740), <3 days
Microscopic observation
Related Products
T3740 Tissue-Clearing Reagent CUBIC-L [for delipidation and decoloring]T3741 Tissue-Clearing Reagent CUBIC-R+ [for RI matching]
C3708 CUBIC-HV™1 3D immunostaining kit
F0622 37% Formaldehyde Solution
H0396 HEPES
| Product Number | C3709 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
Related Laws:
Transport Information:
| H.S.code* | 3822.19-000 |
Application
3D staining and imaging for large specimens
References
- Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues
Articles/Brochures
Product Documents (Note: Some products will not have analytical charts available.)
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