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Products > Glycoscience > Glycoscience TOPICS >

Anti-αGal Polyclonal Antibodies —Antibodies capable of detecting αGal epitope(Galα1-3Gal)—

Anti-αGal antibody exists as a natural antibody in humans. Binding of this antibody to Galαantigens (αGal epitope) expressed on porcine xenograft surfaces are a major factor for determining engraft survival. Recently, it has been observed that therapeutic antibodies and cell processing material for reproductive medicine contain the αGal epitope, which indicates the importance of rapid detection of αGal epitope.

Products

A3123: Anti-αGal Polyclonal Antibody (Chicken)
A3144: Anti-αGal Polyclonal Antibody Biotin Conjugate
A3195: Anti-αGal Chicken Polyclonal Antibody HRP Conjugate

*These antibodies are unavailable in the U.S. and China.

Anti-αGal antibody can be utilized for detection of the αGal epitope on glycoproteins

Western blotting analysis performed using an anti-αGal polyclonalantibody biotin conjugate (A3144).
Lane 1: Thyroglobulin, porcine thyroid gland.
Lane 2: Laminin, Engelbreth-Holm-Swarm murine sarcoma basement membrane.
Lane 3: Thyroglobulin treated with α1-3,4,6 galactosidase.
Lane 4: Laminin treated with α1-3,4,6 galactosidase.

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Anti-αGal antibody shows the same high specificity compared with an anti-αGal monoclonal antibody

Glycoconjugates coated on ELISA plates. Results following epitope and anti-αGal antibodies incubation. 1st Ab were detected using appropriate secondary antibodies.

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